Madzak Catherine
INRA, UMR1319, Micalis Institute, AgroParisTech campus, CBAI, F-78850, Thiverval-Grignon, France,
Appl Microbiol Biotechnol. 2015 Jun;99(11):4559-77. doi: 10.1007/s00253-015-6624-z. Epub 2015 May 7.
The oleaginous yeast Yarrowia lipolytica has become a recognized system for expression/secretion of heterologous proteins. This non-conventional yeast is currently being developed as a workhorse for biotechnology by several research groups throughout the world, especially for single-cell oil production, whole cell bioconversion and upgrading of industrial wastes. This mini-review presents established tools for protein expression in Y. lipolytica and highlights novel developments in the areas of promoter design, surface display, and host strain or metabolic pathway engineering. An overview of the industrial and commercial biotechnological applications of Y. lipolytica is also presented.
产油酵母解脂耶氏酵母已成为一种公认的异源蛋白表达/分泌系统。这种非常规酵母目前正被世界各地的几个研究小组开发成为生物技术的主力军，特别是用于单细胞油脂生产、全细胞生物转化和工业废物升级。这篇小型综述介绍了解脂耶氏酵母中已有的蛋白质表达工具，并突出了启动子设计、表面展示以及宿主菌株或代谢途径工程等领域的新进展。文中还概述了解脂耶氏酵母在工业和商业生物技术方面的应用。
