Shafiei Reza, Sarkari Bahador, Sadjjadi Seyed Mahmoud
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
J Lab Physicians. 2015 Jan-Jun;7(1):17-20. doi: 10.4103/0974-2727.154781.
Serological diagnosis, based on antigenic fractions of the parasite can be used for the early diagnosis of human fascioliasis. The current study aimed to evaluate the efficacy of a 27 kDa immunodominant antigen of Fasciola hepatica adult worms, in an indirect enzyme-linked immunosorbent assay (ELISA) system for serological diagnosis of human fascioliasis.
The immunodiagnosis of human fascioliasis, using a 27 kDa immunodominant antigen, purified from F. hepatica somatic antigens (SAs), was evaluated by Western blotting and ELISA with sera samples of human fascioliasis patients, healthy controls and patients with other parasitic infections.
Using western blotting, from 12 sera of fascioliasis patients, 11 sera (91.6%) detected the 27 kDa subunit. None of 30 samples from healthy controls or 32 sera from nonfascioliasis patients reacted with the 27 kDa antigen. Accordingly, sensitivity and specificity of the system was found to be 91.6% and 100%, respectively. The 27 kDa antigen was purified from the SAs and was used in an indirect ELISA system. Of 15 sera of fascioliasis patients, all (100%) were found to be positive by ELISA whereas only 4 cases (6.25%) of nonfascioliasis patients or healthy controls were false-positive by this system. Accordingly, the sensitivity and specificity of the test were 100% and 93.6%, respectively.
Findings of this study demonstrated that both Western blotting and the indirect ELISA, based on the 27 kDa subunit of F. hepatica SA, are reliable methods for serodiagnosis of human fascioliasis.
基于寄生虫抗原成分的血清学诊断可用于人类肝片吸虫病的早期诊断。本研究旨在评估肝片吸虫成虫27 kDa免疫显性抗原在间接酶联免疫吸附测定（ELISA）系统中用于人类肝片吸虫病血清学诊断的效果。
使用从肝片吸虫体抗原（SAs）中纯化的27 kDa免疫显性抗原，通过蛋白质印迹法和ELISA，对人类肝片吸虫病患者、健康对照者以及其他寄生虫感染患者的血清样本进行人类肝片吸虫病免疫诊断评估。
通过蛋白质印迹法，在12份肝片吸虫病患者血清中，有11份血清（91.6%）检测到27 kDa亚基。30份健康对照样本或32份非肝片吸虫病患者血清中无一与27 kDa抗原发生反应。因此，该系统的敏感性和特异性分别为91.6%和100%。从SAs中纯化出27 kDa抗原，并将其用于间接ELISA系统。在15份肝片吸虫病患者血清中，通过ELISA检测发现所有样本（100%）均为阳性，而在非肝片吸虫病患者或健康对照者中，只有4例（6.25%）被该系统误判为阳性。因此，该检测的敏感性和特异性分别为100%和93.6%。
本研究结果表明，基于肝片吸虫SAs的27 kDa亚基的蛋白质印迹法和间接ELISA都是人类肝片吸虫病血清学诊断的可靠方法。
