Department of Physiological Sciences, School of Dentistry of Piracicaba, University of Campinas, Avenue Limeira 901, 13414 903 Piracicaba, SP, Brazil.
Department of Agro-Food Industry, Food and Nutrition, "Luiz de Queiroz" College of Agriculture, University of São Paulo, Avenue Pádua Dias 11, 13418-900 Piracicaba, SP, Brazil.
Evid Based Complement Alternat Med. 2015;2015:459846. doi: 10.1155/2015/459846. Epub 2015 Apr 8.
The aim of this study was to evaluate the gastroprotective activity of ethanolic extract of geopropolis (EEGP) from Melipona scutellaris and to investigate the possible mechanisms of action. The gastroprotective activity of the EEGP was evaluated using model ulcer induced by ethanol. To elucidate the possible mechanisms of action, we investigated the involvement of the nonprotein sulfhydryl (NP-SH) groups, nitric oxide and prostaglandins. In addition, the antisecretory activity of EEGP was also evaluated by pylorus ligated model. The EEGP orally administrated (300 mg/kg) reduced the ulcerative lesions induced by the ethanol (P < 0.05). Regarding the mechanism of action, the prior administration of nitric oxide and prostaglandins antagonists suppressed the activity of gastroprotective EEGP (P < 0.05). On the other hand the gastroprotective activity of EEGP was kept in the group pretreated with the antagonist of the NP-SH groups; furthermore the antisecretory activity was not significant (P > 0.05). These results support the alternative medicine use of geopropolis as gastroprotective and the activities observed show to be related to nitric oxide and prostaglandins production.
本研究旨在评估 Melipona scutellaris 蜂胶乙醇提取物（EEGP）的胃保护活性，并探讨其可能的作用机制。采用乙醇诱导的溃疡模型评价 EEGP 的胃保护活性。为阐明可能的作用机制，我们研究了非蛋白巯基（NP-SH）基团、一氧化氮和前列腺素的参与情况。此外，还通过幽门结扎模型评价了 EEGP 的抗分泌活性。EEGP 口服（300mg/kg）可减轻乙醇诱导的溃疡损伤（P<0.05）。关于作用机制，一氧化氮和前列腺素拮抗剂的预先给予抑制了胃保护 EEGP 的活性（P<0.05）。另一方面，NP-SH 组拮抗剂预处理组的 EEGP 胃保护活性不受影响；此外，抗分泌活性不显著（P>0.05）。这些结果支持将蜂胶作为胃保护剂的替代医学用途，并且观察到的活性与一氧化氮和前列腺素的产生有关。
