Department of Physiological Sciences, Dentistry School of Piracicaba, State University of Campinas (UNICAMP), Avenida Limeira 901, 13414-903 Piracicaba, SP, Brazil.
Evid Based Complement Alternat Med. 2013;2013:256287. doi: 10.1155/2013/256287. Epub 2013 Jun 13.
The aim of this study was to evaluate the influence of the bioactive nonpolar fraction of geopropolis on Streptococcus mutans biofilm. The ethanolic extract of Melipona scutellaris geopropolis was subjected to a liquid-liquid partition, thus obtaining the bioactive hexane fraction (HF) possessing antimicrobial activity. The effects of HF on S. mutans UA159 biofilms generated on saliva-coated hydroxyapatite discs were analyzed by inhibition of formation, killing assay, and glycolytic pH-drop assays. Furthermore, biofilms treated with vehicle control and HF were analyzed by scanning electron microscopy (SEM). HF at 250 μ g/mL and 400 μ g/mL caused 38% and 53% reduction in the biomass of biofilm, respectively, when compared to vehicle control (P < 0.05) subsequently observed at SEM images, and this reduction was noticed in the amounts of extracellular alkali-soluble glucans, intracellular iodophilic polysaccharides, and proteins. In addition, the S. mutans viability (killing assay) and acid production by glycolytic pH drop were not affected (P > 0.05). In conclusion, the bioactive HF of geopropolis was promising to control the S. mutans biofilm formation, without affecting the microbial population but interfering with its structure by reducing the biochemical content of biofilm matrix.
本研究旨在评估天然没药生物活性非极性部位对变形链球菌生物膜的影响。采用液-液萃取法对 M. scutellaris 天然没药的乙醇提取物进行分离，得到具有抗菌活性的正己烷部位（HF）。采用生成抑制、杀菌试验和糖酵解 pH 值下降试验分析 HF 对唾液包被羟磷灰石片上 S. mutans UA159 生物膜的影响。用扫描电子显微镜（SEM）分析经载剂和 HF 处理的生物膜。与载剂组相比，HF 在 250 μ g/mL 和 400 μ g/mL 时分别使生物膜生物量减少 38%和 53%（P<0.05），SEM 图像也观察到了这一结果，并且细胞外可溶碱性葡聚糖、细胞内嗜碘多糖和蛋白质的含量也减少。此外，HF 对 S. mutans 的活力（杀菌试验）和糖酵解 pH 值下降产生的酸没有影响（P>0.05）。总之，天然没药的生物活性 HF 有望控制 S. mutans 生物膜的形成，而不会影响微生物种群，而是通过降低生物膜基质的生化含量来干扰其结构。
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