School of Public Health, Jilin University, Changchun, 130021, P. R. China.
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
Macromol Biosci. 2015 Aug;15(8):1070-80. doi: 10.1002/mabi.201500069. Epub 2015 May 7.
In this study, insulin-like growth factor 1 (IGF-1) was successfully immobilized on the poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) and pure PLGA microcarriers via polydopamine (pDA). The results demonstrated that the pDA layer facilitated simple and highly efficient immobilization of peptides on the microcarriers within 20 min. Mouse adipose-derived stem cells (ADSCs) attachment and proliferation on IGF-1-immobilized microcarriers were much higher than non-immobilized ones. More importantly, the IGF-1-immobilized PLGA/HA microcarriers significantly increased alkaline phosphatase (ALP) activity and expression of osteogenesis-related genes of ADSCs. Therefore, it is considered that the IGF-1-decorated PLGA/HA microcarriers will be of great value in the bone tissue engineering.
在这项研究中，胰岛素样生长因子 1 (IGF-1) 通过聚多巴胺 (pDA) 成功固定在聚（丙交酯-共-乙交酯）/羟基磷灰石 (PLGA/HA) 和纯 PLGA 微载体上。结果表明，pDA 层在 20 分钟内促进了肽在微载体上的简单和高效固定。固定 IGF-1 的微载体上的小鼠脂肪来源干细胞 (ADSCs) 附着和增殖明显高于非固定的微载体。更重要的是，固定 IGF-1 的 PLGA/HA 微载体显著增加了 ADSCs 的碱性磷酸酶 (ALP) 活性和骨生成相关基因的表达。因此，认为 IGF-1 修饰的 PLGA/HA 微载体在骨组织工程中具有重要价值。
