Larsen P N, Moesgaard F, Gottrup F, Helledie N
Department of Surgical Gastroenterology C, Rigshospitalet, University Hospital, Copenhagen, Denmark.
Scand J Clin Lab Invest. 1989 Oct;49(6):513-9. doi: 10.3109/00365518909089130.
A new oxygen tonometry system consisting of a silicone tube, highly permeable to O2 and CO2 is described. The silicone tube was connected to a membrane-covered transcutaneous oxygen electrode (E5242 Radiometer A/S, Copenhagen, Denmark) via an airtight polycarbonate chamber, and flushed with isotonic saline equilibrated with atmospheric air. The present tonometer system offers certain advantages compared with other systems: continuous reading, minimal oxygen consumption, furthermore the system is thermostated and is insensitive to movement. The tonometry system was tested in vitro for characterization of a silicone tube (Coroplast, Fritz Müller KG, Wuppertal, FRG) 1.0 mm in inner diameter and 1.5 mm in outer diameter. The experiments showed that the oxygen tension measured at the electrode after passage of the tonometer approached the oxygen tension outside the tonometer when the length of the tonometer was increased and when the flushing rate of saline through the tonometer was decreased. The time taken for the flushing solution to reach an equilibrium with the oxygen tension outside the tonometer increased with increasing tonometer length, and decreased with increasing flushing rate. Changing the difference between oxygen partial pressure in the flushing solution and the oxygen partial pressure outside the tonometer tube did not influence the relative equilibration value and the equilibration time. When a pO2 value is measured by the electrode, the exact oxygen tension outside the tonometer tube, for every given length of the tonometer and flushing rate through the tonometer can be read from our calibration curves.
本文描述了一种新型氧张力测量系统，该系统由对氧气和二氧化碳具有高渗透性的硅胶管组成。硅胶管通过一个气密的聚碳酸酯腔室连接到一个覆盖有膜的经皮氧电极（E5242，Radiometer A/S，丹麦哥本哈根），并用与大气平衡的等渗盐水冲洗。与其他系统相比，本张力测量系统具有一定优势：读数连续、氧气消耗极少，此外该系统具有恒温功能且对运动不敏感。对内径1.0毫米、外径1.5毫米的硅胶管（Coroplast，Fritz Müller KG，德国伍珀塔尔）进行体外测试以表征该张力测量系统。实验表明，当增加张力测量仪的长度并降低盐水通过张力测量仪的冲洗速率时，张力测量仪通过后在电极处测得的氧张力接近张力测量仪外部的氧张力。冲洗溶液与张力测量仪外部氧张力达到平衡所需的时间随张力测量仪长度增加而增加，随冲洗速率增加而减少。改变冲洗溶液中的氧分压与张力测量仪管外部氧分压之间的差值不会影响相对平衡值和平衡时间。当通过电极测量pO2值时，对于张力测量仪的每个给定长度和通过张力测量仪的冲洗速率，可以从我们的校准曲线中读取张力测量仪管外部的确切氧张力。
