Larsen P N, Pedersen I, Moesgaard F
Department of Surgical Gastroenterology C, Rigshospitalet, University Hospital, Copenhagen, Denmark.
Scand J Clin Lab Invest. 1993 Jul;53(4):317-25. doi: 10.3109/00365519309086622.
A new tonometry system for continuous and synchronous measurement of tissue oxygen- and carbon dioxide tension is described and characterized in vitro. The tonometer system consists of an O2 and CO2 permeable silicone tube continuously flushed with isotonic saline by an injection pump. When the saline passes through the tonometer tube it equilibrates with O2 and CO2 outside the tube. The oxygen- and carbon dioxide tension of the flushing solution after passage of the tonometer tube are measured by a transcutaneous combined oxygen/carbon dioxide electrode (E5280 Radiometer A/S, Copenhagen, Denmark), connected to the tonometer tube via an airtight polycarbonate chamber. In order to characterize the tonometer system in vitro the tonometer tube was submerged in a test chamber containing isotonic saline, 33 degrees C to 41 degrees C, with varying partial pressures of O2 and CO2. For various lengths of the tonometer and flushing rates through the tonometer the partial pressures of oxygen and carbon dioxide in the flushing solution (pO2eq and pCO2eq), after passage through the tonometer were recorded and compared to the known partial pressures of oxygen and carbon dioxide in the test chamber solution (pO2 test and pCO2test). PO2eq and pCO2eq approached pO2test and pCO2test, when the length of the tonometer was increased, and the flushing rate through the tonometer was decreased. The relative differences (D) between pO2eq and pCO2eq at the one hand and pO2test and pCO2test at the other hand were calculated, and equilibration curves were constructed.(ABSTRACT TRUNCATED AT 250 WORDS)
本文描述了一种用于连续同步测量组织氧分压和二氧化碳分压的新型眼压测量系统，并在体外对其进行了特性研究。眼压测量系统由一根可透过氧气和二氧化碳的硅胶管组成，该硅胶管由注射泵用等渗盐水持续冲洗。当盐水通过眼压测量管时，它会与管外的氧气和二氧化碳达到平衡。通过眼压测量管后的冲洗液的氧分压和二氧化碳分压，由一个经皮联合氧/二氧化碳电极（丹麦哥本哈根Radiometer A/S公司的E5280型）测量，该电极通过一个气密的聚碳酸酯腔室与眼压测量管相连。为了在体外对眼压测量系统进行特性研究，将眼压测量管浸入一个装有温度在33℃至41℃之间、氧分压和二氧化碳分压不同的等渗盐水的测试腔室中。对于不同长度的眼压测量管和通过眼压测量管的冲洗速率，记录通过眼压测量管后冲洗液中的氧分压和二氧化碳分压（pO2eq和pCO2eq），并与测试腔室溶液中已知的氧分压和二氧化碳分压（pO2test和pCO2test）进行比较。当眼压测量管的长度增加且通过眼压测量管的冲洗速率降低时，pO2eq和pCO2eq接近pO2test和pCO2test。计算了pO2eq和pCO2eq与pO2test和pCO2test之间的相对差异（D），并绘制了平衡曲线。（摘要截取自250字）
