Matysiak Joanna, Matuszewska Eliza, Kowalski Marek L, Kosiński Sławomir W, Smorawska-Sabanty Ewa, Matysiak Jan
Faculty of Health Sciences, The President Stanisław Wojciechowski State University of Applied Sciences in Kalisz, 62-800 Kalisz, Poland.
Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 60-780 Poznań, Poland.
Vaccines (Basel). 2021 Mar 12;9(3):249. doi: 10.3390/vaccines9030249.
Venom immunotherapy (VIT) is administered to allergic patients to reduce the risk of dangerous systemic reactions following an insect sting. To better understand the mechanism of this treatment and its impact on the human organism, we analysed serum proteomic patterns obtained at five time-points from -venom-allergic patients undergoing VIT. For statistical analyses, patients were additionally divided into two groups (high responders and low responders) according to serum sIgG4 levels. VIT was found to be associated with changes in seven proteins: the fibrinogen alpha chain, complement C4-A, complement C3, filamin-B, kininogen-1, myosin-9 and inter-alpha-trypsin inhibitor heavy chain H1. The number of discriminative (mass-to-charge ratio) features increased up to the 90th day of VIT, which may be associated with the development of immunity after the administration of increased venom doses. It may also suggest that during VIT, there may occur processes involved not only in protein synthesis but also in protein degradation (caused by proteolytic venom components). The results are consistent with measured serum sIgG4 levels, which increased from 2.04 mgA/I at baseline to 7.25 mgA/I at 90 days. Moreover, the major proteomic changes were detected separately in the high responder group. This may suggest that changes in protein-peptide profiles reflect the actual response to VIT.
毒液免疫疗法（VIT）用于治疗过敏患者，以降低昆虫叮咬后发生危险全身反应的风险。为了更好地理解这种治疗的机制及其对人体的影响，我们分析了接受VIT的毒液过敏患者在五个时间点获得的血清蛋白质组学模式。为了进行统计分析，根据血清sIgG4水平将患者额外分为两组（高反应者和低反应者）。发现VIT与七种蛋白质的变化有关：纤维蛋白原α链、补体C4-A、补体C3、细丝蛋白-B、激肽原-1、肌球蛋白-9和间α胰蛋白酶抑制剂重链H1。在VIT的第90天之前，鉴别性（质荷比）特征的数量增加，这可能与增加毒液剂量后免疫力的发展有关。这也可能表明，在VIT期间，可能不仅发生蛋白质合成过程，还发生蛋白质降解过程（由蛋白水解性毒液成分引起）。结果与测得的血清sIgG4水平一致，该水平从基线时的2.04mgA/I增加到90天时的7.25mgA/I。此外，在高反应者组中分别检测到主要的蛋白质组学变化。这可能表明蛋白质-肽谱的变化反映了对VIT的实际反应。
