Cao Huan, Chen Ming-Mao, Liu Yan, Liu Yuan-Yuan, Huang Yu-Qing, Wang Jian-Hua, Chen Jing-Di, Zhang Qi-Qing
Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
Colloids Surf B Biointerfaces. 2015 Dec 1;136:1098-106. doi: 10.1016/j.colsurfb.2015.10.022. Epub 2015 Oct 23.
To design a scaffold controlled release system for skin tissue engineering, fish collagen/chitosan/chondroitin sulfate scaffolds were fabricated by freeze-drying and incorporated with bFGF-loaded PLGA microspheres (MPs). SEM showed that the scaffolds exhibited an interconnected porous structure, and the spherical MPs were uniformly distributed into the scaffolds. The higher swelling and degradation rate of scaffolds/MPs could lead to a higher diffusion rate of MPs from the scaffolds, causing an increase in the protein release. The release rate of proteins could be adjusted by the size of MPs and the ratio of collagen to chitosan of scaffolds. Circular dichroism spectroscopy and MTT of bFGF after release indicated that the released bFGF retained its structural integrity and bioactivity during preparation. Cell proliferation and in vivo evaluation results suggested that the scaffolds/MPs had a good biocompatibility and an ability to promote fibroblast cell proliferation and skin tissue regeneration. These results demonstrated that this scaffold/MP controlled release system has the potential for skin tissue engineering.
为设计用于皮肤组织工程的支架控释系统，通过冷冻干燥制备了鱼胶原蛋白/壳聚糖/硫酸软骨素支架，并将负载碱性成纤维细胞生长因子（bFGF）的聚乳酸-羟基乙酸共聚物（PLGA）微球（MPs）掺入其中。扫描电子显微镜（SEM）显示，支架呈现出相互连通的多孔结构，球形MPs均匀分布于支架中。支架/MPs较高的溶胀和降解速率可导致MPs从支架中的扩散速率更高，从而使蛋白质释放增加。蛋白质的释放速率可通过MPs的大小以及支架中胶原蛋白与壳聚糖的比例进行调节。释放后bFGF的圆二色光谱和MTT检测表明，所释放的bFGF在制备过程中保持了其结构完整性和生物活性。细胞增殖和体内评估结果表明，支架/MPs具有良好的生物相容性以及促进成纤维细胞增殖和皮肤组织再生的能力。这些结果证明，这种支架/MP控释系统具有用于皮肤组织工程的潜力。
