Pimenta Hévelin Couto, Borges Álvaro Henrique, Bandeca Matheus Coelho, Neves Ana Thereza Sabóia, Fontes Rodrigo Gusmão, da Silva Priscila Vieira, Aranha Andreza Maria Fábio
Professor, Department of Pediatric Dentistry, School of Dentistry, University of Cuiabá- MT, Brazil.
Associate Professor, Department of Post-Graduate Program Integrated Dentistry Sciences, School of Dentistry, University of Cuiabá-MT, Brazil.
J Int Oral Health. 2015 Apr;7(4):54-7.
The aim of this study was to investigate the antibacterial activity of pulp capping materials used in primary teeth (formocresol [FC], zinc oxide and eugenol cement [ZOE], ZOE mixed with FC [ZOEFC], mineral trioxide aggregate [MTA] and calcium hydroxide [CH]) against cariogenic bacteria. The agar plate diffusion test was used for the cultures, including saline solution as a negative control. A base layer of 15 mL of brain heart infusion agar was inoculated with 300 mL of each inoculum. Twelve wells were made and completely filled with one of the testing materials for each bacteria strain. The plates were incubated at 37°C for 48 h. Zones of microbial inhibition and material diffusion were measured and photographed. The results obtained were analyzed by Kruskal-Wallis and Mann-Whitney non-parametric tests. Respectively, the medium zones of bacteria inhibition of FC, ZOE, ZOEFC, MTA and CH against Streptococcus mutans growth were 28.5, 15.2, 20.8, 9.3 and 11.6; against Lactobacillus acidophilus growth were 28.7, 14.8, 15.3, 15.2 and 20.0, and against Actinomyces viscosus growth were 13.6, 13.5, 14.7, 10.0 and 13.6. We might confirmed the high antibacterial activity of FC solution, especially against S. mutans and L. acidophilus, as wells as, the low inhibitory effect of MTA cement on the cariogenic bacteria studied.
本研究旨在调查用于乳牙的盖髓材料（甲醛甲酚[FC]、氧化锌丁香酚水门汀[ZOE]、ZOE与FC混合[ZOEFC]、三氧化矿物凝聚体[MTA]和氢氧化钙[CH]）对致龋菌的抗菌活性。采用琼脂平板扩散试验进行培养，包括用生理盐水溶液作为阴性对照。在15 mL脑心浸液琼脂的基层中接种300 μL每种接种物。制作12个孔，并为每种细菌菌株用一种测试材料完全填满。平板在37°C下孵育48小时。测量并拍摄微生物抑制区和材料扩散区。所得结果通过Kruskal-Wallis和Mann-Whitney非参数检验进行分析。FC、ZOE、ZOEFC、MTA和CH对变形链球菌生长的细菌抑制中区分别为28.5、15.2、20.8、9.3和11.6；对嗜酸乳杆菌生长的细菌抑制中区分别为28.7、14.8、15.3、15.2和20.0，对粘性放线菌生长的细菌抑制中区分别为13.6、13.5、14.7、10.0和13.
