Department of Operative Dentistry, University of Pavia, Piazzale Golgi 3, Pavia, Italy.
Dent Traumatol. 2012 Jun;28(3):205-9. doi: 10.1111/j.1600-9657.2011.01074.x. Epub 2011 Nov 3.
The objective of this study was to compare in vitro the antibacterial activity of Tetraclean (mixture of doxycycline, citric acid and polypropylene glycol), Niclor 5 (5.25% sodium hypochlorite solution), Cloreximid (0.2% chlorhexidine and 0.2% cetrimide solution) and hydrogen peroxide 12 volumes on three endodontic pathogens associated with primary endodontic infections. Enterococcus faecalis, Streptococcus mutans and Staphylococcus aureus strains were selected to evaluate the antibacterial activity of endodontic irrigants by the agar disc-diffusion test.
Paper discs were saturated with each one of the test solutions (at room temperature and preheating at 50°C) and placed onto culture agar-plates preadsorbed with bacterial cells and further incubated for 24 h at 37°C. The growth inhibition zones around each irrigant were recorded and compared for each bacterial strain. Kruskal-Wallis and Mann-Whitney tests were applied to compare the various groups.
At room temperature, Tetraclean showed significantly higher inhibition of bacteria growth than all other irrigants tested. Preheating at 50°C significantly increased growth inhibition for all the groups tested. At 50°C, hydrogen peroxide 12 volumes and Tetraclean showed significantly higher efficacy than all other irrigants tested.
50°C-preheated hydrogen peroxide 12 volumes and Tetraclean showed highest inhibition of the bacterial growth.
本研究旨在比较 Tetraclean（强力霉素、柠檬酸和聚丙二醇的混合物）、Niclor 5（5.25%次氯酸钠溶液）、Cloreximid（0.2%洗必泰、0.2%西曲溴铵溶液）和 12 倍过氧化氢在体外对与原发性牙髓感染相关的三种牙髓病原体的抗菌活性。选择粪肠球菌、变形链球菌和金黄色葡萄球菌菌株，通过琼脂碟扩散试验评估牙髓冲洗剂的抗菌活性。
将每个测试溶液（在室温下和预热至 50°C）的纸碟饱和，并将其放置在预先吸附有细菌细胞的培养琼脂板上，然后在 37°C 下进一步孵育 24 小时。记录每个冲洗剂周围的抑菌区，并比较每种细菌菌株的抑菌区。应用 Kruskal-Wallis 和 Mann-Whitney 检验比较各组之间的差异。
在室温下，Tetraclean 对细菌生长的抑制作用明显高于所有其他测试的冲洗剂。50°C 预热显著增加了所有测试组的抑菌作用。在 50°C 时，12 倍过氧化氢和 Tetraclean 比所有其他测试的冲洗剂显示出更高的疗效。
50°C 预热的 12 倍过氧化氢和 Tetraclean 对细菌生长的抑制作用最强。
