Mony Bejoy, Ebenezar A V Rajesh, Ghani Mohamed Fayas, Narayanan Ashwin, S Anand, Mohan Ajit George
Senior Lecturer, Department of Conservative Dentistry & Endodontics, Tagore Dental College & Hospital , Chennai, India .
Professor, Department of Conservative Dentistry & Endodontics, Madha Dental College & Hospital , Chennai, India .
J Clin Diagn Res. 2015 Mar;9(3):ZC30-2. doi: 10.7860/JCDR/2015/11404.5656. Epub 2015 Mar 1.
To evaluate the remineralization potential of enamel surface lesion using chicken eggshell powder (CESP) solution.
Ten freshly extracted un-erupted third molars decoronated at cement-enamel junction (CEJ) used in this study. Each decoronated tooth was sectioned to get 4 samples of enamel blocks such that 40 blocks were obtained which were then subjected to demineralization protocol and grouped as: Group 1-untreated group, Group 2-subsurface demineralization, Group 3-subsurface demineralization + 7 days CESP immersion, Group 4- subsurface demineralization + clinpro application. The samples were evaluated for X-ray fluorescence spectroscopy analysis, microhardness testing and atomic analyses using energy dispersive X-ray spectroscopy. The data were statistically analysed by using one-way ANOVA and Tukey - Kramer multiple comparison test.
X-ray Fluorescence spectroscopy shows Calcium concentration of 98% and 0.46% of phosphate. Group 4 (Clinpro) shows the highest efficacy in enhancing the remineralization followed by Group 3 (CESP). The results of atomic analyses showed that quantitative amounts of Ca weight % and P weight % is statistically greater for all the three groups except the demineralized group.
CESP with higher calcium content can remineralise enamel surface lesion.
使用鸡蛋壳粉(CESP)溶液评估牙釉质表面病变的再矿化潜力。
本研究使用了10颗刚拔除的未萌出第三磨牙,在牙骨质-牙釉质界(CEJ)处去除牙冠。将每颗去除牙冠的牙齿进行切片,得到4个牙釉质块样本,共获得40个样本,然后对其进行脱矿处理,并分组如下:第1组为未处理组,第2组为表层下脱矿组,第3组为表层下脱矿 + 7天CESP浸泡组,第4组为表层下脱矿 + Clinpro应用组。使用能量色散X射线光谱仪对样本进行X射线荧光光谱分析、显微硬度测试和元素分析。数据采用单因素方差分析和Tukey - Kramer多重比较检验进行统计学分析。
X射线荧光光谱显示钙浓度为98%,磷浓度为0.46%。第4组(Clinpro)在促进再矿化方面显示出最高的功效,其次是第3组(CESP)。元素分析结果表明,除脱矿组外,其他三组的钙重量百分比和磷重量百分比的定量在统计学上均更高。
钙含量较高的CESP可使牙釉质表面病变再矿化。