用鸡蛋壳浆液对牙釉质和牙本质进行仿生再矿化：一项研究。

Biomimetic remineralization of enamel and dentin with chicken eggshell slurry: An study.

作者信息

Jain Nainy, Devadiga Darshana, Rao Rahul D, Anumula Lavanya, Shetty Sameep, Ingle Aditya

机构信息

Department of Conservative Dentistry and Endodontics, A B Shetty Memorial Institute of Dental Sciences, NITTE (Deemed to be University), Mangalore, Karnataka, India.

Department of Conservative Dentistry and Endodontics, Bharti Vidyapeeth Deemed to be University Dental College and Hospital, Navi Mumbai, Maharashtra, India.

出版信息

J Conserv Dent Endod. 2025 Apr;28(4):349-354. doi: 10.4103/JCDE.JCDE_67_25. Epub 2025 Apr 3.

DOI:10.4103/JCDE.JCDE_67_25

PMID:40302829

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12037129/

Abstract

CONTEXT

In the light of escalating focus on preventive and minimally invasive dental practices, this study explores the potential of chicken eggshell powder (CESP) as an eco-friendly source of calcium and bioactive compounds for remineralization of demineralized dental substrates.

AIMS

The aims of this study were to characterize and evaluate the remineralization potential of CESP slurry on demineralized human permanent teeth.

SUBJECTS AND METHODS

CESP was prepared from chicken eggshell using calcination procedure followed which CESP slurry was prepared. The CESP powder was characterized using Fourier transform infrared spectroscopy (FTIR) analysis. Twenty-four intact human third molars were collected and sectioned, randomly allocated into two groups ( = 96): G1 - enamel (E) and G2 - dentin (D) which were further divided into the following six subgroups ( = 16) based on the intended surface treatment: E1 (sound enamel-control), E2 (demineralized enamel with no treatment), and E3 (enamel treated with CESP), D1 (sound dentin-control), D2 (demineralized dentin with no treatment), and D3 (dentin treated with CESP). All enamel and dentin blocks except those in Group E1 and D1 were immersed in a demineralizing solution for 72 h. E3 and D3 were subjected to the application of CESP slurry twice daily for a week by gentle rubbing for 2 min. All samples underwent pH-cycling and were tested using a Vickers hardness tester and scanning electron microscopy analysis.

STATISTICAL ANALYSIS USED

Tukey's honestly significant difference test and paired samples test were used for statistical analysis ( < 0.005).

RESULTS

FTIR analysis of CESP revealed notable peaks in the range of 1400-1500 cm and sharp peaks around 1000-700 cm, a feature commonly observed in calcium carbonate. A significantly higher mean % recovery of surface microhardness (%RSMH) was seen as compared to the mean % decrease in surface microhardness (%DSMH) in enamel with a mean difference ofcm-13.32 ± 4.0 ( = 0.000), whereas, in the case of dentin, a significantly higher mean % DSMH was seen as compared to mean % RSMH with a mean difference of 61.47 ± 3.7 ( = 0.000).

CONCLUSIONS

The elevated pH of the chicken eggshell solution, coupled with the abundant bioavailable calcium content, holds the potential to favor remineralization. The findings suggest that the application of CESP slurry could contribute to the remineralization process.

摘要

背景

鉴于对预防性和微创牙科实践的关注度不断提高，本研究探索了鸡蛋壳粉（CESP）作为一种生态友好型钙源和生物活性化合物来源，用于脱矿牙体基质再矿化的潜力。

目的

本研究的目的是表征和评估CESP浆液对脱矿人恒牙的再矿化潜力。

对象与方法

采用煅烧工艺从鸡蛋壳制备CESP，然后制备CESP浆液。使用傅里叶变换红外光谱（FTIR）分析对CESP粉末进行表征。收集24颗完整的人第三磨牙并进行切片，随机分为两组（每组n = 96）：G1 - 牙釉质（E）组和G2 - 牙本质（D）组，根据预期的表面处理进一步分为以下六个亚组（每组n = 16）：E1（健康牙釉质 - 对照组）、E2（未处理的脱矿牙釉质）和E3（用CESP处理的牙釉质），D1（健康牙本质 - 对照组）、D2（未处理的脱矿牙本质）和D3（用CESP处理的牙本质）。除E1和D1组外，所有牙釉质和牙本质块均浸入脱矿溶液中72小时。E3和D3组每天两次通过轻柔摩擦2分钟施加CESP浆液，持续一周。所有样本进行pH循环，并使用维氏硬度测试仪和扫描电子显微镜分析进行测试。

所用统计分析方法

采用Tukey's真实显著差异检验和配对样本检验进行统计分析（P < 0.005）。

结果

CESP的FTIR分析显示在1400 - 1500 cm范围内有明显峰值，在1000 - 700 cm左右有尖锐峰值，这是碳酸钙中常见的特征。与牙釉质表面显微硬度平均降低百分比（%DSMH）相比，表面显微硬度平均恢复百分比（%RSMH）显著更高，平均差值为13.32 ± 4.0（P = 0.000）；而在牙本质的情况下，与平均%RSMH相比，平均%DSMH显著更高，平均差值为61.47 ± 3.7（P = 0.000）。