Wan Juan, Laliberté Jean-François
a INRS-Institut Armand-Frappier ; Laval; Québec , Canada.
Plant Signal Behav. 2015;10(8):e1042639. doi: 10.1080/15592324.2015.1042639.
It is generally accepted that in order to establish a systemic infection in a plant, viruses move from the initially infected cell to the vascular tissues by cell-to-cell movement through plasmodesmata (PD), and load into the vascular conducting tubes (i.e. phloem sieve elements and xylem vessel elements) for long-distance movement. The viral unit in these movements can be a virion or a yet-to-be-defined ribonucleic protein (RNP) complex. Using live-cell imaging, our laboratory has previously demonstrated that membrane-bound replication complexes move cell-to-cell during turnip mosaic virus (TuMV) infection. Our recent study shows that these membrane-bound replication complexes end up in the vascular conducting tubes, which is likely the case for potato virus X (PVX) also. The presence of TuMV-induced membrane complexes in xylem vessels suggests that viral components could also be found in other apoplastic regions of the plant, such as the intercellular space. This possibility may have implications regarding how we approach the study of plant innate immune responses against viruses.
一般认为，为了在植物中建立系统性感染，病毒通过胞间连丝进行细胞间移动，从最初感染的细胞移动到维管组织，并装载到维管传导管（即韧皮部筛管分子和木质部导管分子）中进行长距离移动。这些移动过程中的病毒单位可以是病毒粒子或尚未确定的核糖核蛋白（RNP）复合体。利用活细胞成像技术，我们实验室之前已经证明，膜结合复制复合体在芜菁花叶病毒（TuMV）感染期间进行细胞间移动。我们最近的研究表明，这些膜结合复制复合体最终存在于维管传导管中，马铃薯X病毒（PVX）可能也是如此。木质部导管中存在TuMV诱导的膜复合体表明，病毒成分也可能存在于植物的其他质外体区域，如细胞间隙。这种可能性可能对我们研究植物针对病毒的先天免疫反应的方式有影响。
