Buiting A G, Thompson J, van der Keur D, Schmal-Bauer W C, Bertina R M
Department of Infectious Diseases, University Hospital, Leiden, The Netherlands.
Thromb Haemost. 1989 Nov 24;62(3):1029-33.
To investigate activation of the coagulation system in bacterial endocarditis, we determined the procoagulant activity of blood monocytes isolated from rabbits with Streptococcus sanguis-infected or sterile catheter-induced endocardial vegetations. This activity was determined directly after isolation from the peripheral blood and after stimulation in vitro by either endotoxin or by phagocytosis of S. sanguis. The procoagulant activity of the vegetations of these rabbits was also determined. The procoagulant activity of blood monocytes of rabbits with S. sanguis endocarditis was found to be similar to the activity of monocytes of rabbits with sterile vegetations, both at the time of isolation and after stimulation in vitro by exposure to endotoxin or phagocytosis of bacteria. The procoagulant activity of infected vegetations was significantly higher than that of sterile vegetations. We conclude that in bacterial endocarditis the coagulation system is activated locally at the site of the vegetation. Triggering probably occurs by thromboplastin generated by monocytes activated by phagocytosis of bacteria on the vegetational surface.
为研究细菌性心内膜炎中凝血系统的激活情况，我们测定了从患有血链球菌感染性或无菌导管诱导性心内膜赘生物的家兔中分离出的血液单核细胞的促凝活性。该活性在从外周血中分离后以及在体外经内毒素刺激或吞噬血链球菌后直接进行测定。还测定了这些家兔赘生物的促凝活性。发现患有血链球菌性心内膜炎的家兔血液单核细胞的促凝活性在分离时以及在体外经内毒素暴露或细菌吞噬刺激后，与患有无菌赘生物的家兔单核细胞的活性相似。感染性赘生物的促凝活性明显高于无菌赘生物。我们得出结论，在细菌性心内膜炎中，凝血系统在赘生物部位局部被激活。触发可能是由赘生物表面细菌吞噬激活的单核细胞产生的凝血酶原引发的。
