Department of Pathophysiology-Periodontal Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Okayama, Japan.
Ministry of Health, Labour and Welfare Medical Politics Economic Section, Medical Equipment Policy Office, Tokyo, Japan.
J Periodontol. 2019 Sep;90(9):1043-1052. doi: 10.1002/JPER.18-0674. Epub 2019 Apr 14.
Basic fibroblast growth factor (bFGF) has been applied for periodontal regeneration. However, the application depends on bone defect morphology because bFGF diffuses rapidly from defect sites. In a previous study, collagen-binding bFGF (CB-bFGF) has been shown to enhance bone formation by collagen-anchoring in the orthopedic field. The aim of this study is to demonstrate the efficacy of CB-bFGF with collagen scaffolds in bone regeneration of horizontal bone defect.
Cell proliferation activity and collagen binding activity of CB-bFGF was confirmed by WST-8 assay and collagen binding assay, respectively. The retention of CB-bFGF in the collagen sheet (CS) was measured by fluorescence imaging. The rat horizontal alveolar bone defect model was employed to investigate the efficacy of CB-bFGF with collagen powder (CP). After 4 and 8 weeks, the regenerative efficacy was evaluated by microcomputed tomography, histological, and immunohistochemical analyses.
CB-bFGF had a comparable proliferation activity to bFGF and a collagen binding activity. CB-bFGF was retained in CS longer than bFGF. At 8 weeks postoperation, bone volume, bone mineral content, and new bone area in CB-bFGF/CP group were significantly increased compared with those in other groups. Furthermore, epithelial downgrowth was significantly suppressed in CB-bFGF/CP group. At 4 weeks, the numbers of osteocalcin, proliferating cell nuclear antigen, and osteopontin-positive cells at the regeneration site in CB-bFGF/CP group were greater than those in other groups.
CB-bFGF/CP effectively promoted bone regeneration of horizontal bone defect possibly by sustained release of bFGF. The potential of CB-bFGF composite material for improved periodontal regeneration in vertical axis was shown.
碱性成纤维细胞生长因子（bFGF）已被应用于牙周再生。然而，由于 bFGF 会从缺损部位迅速扩散，因此其应用取决于骨缺损的形态。在之前的研究中，已证实胶原结合碱性成纤维细胞生长因子（CB-bFGF）通过在骨科领域与胶原结合锚定可增强骨形成。本研究旨在证明 CB-bFGF 与胶原支架在水平骨缺损骨再生中的功效。
通过 WST-8 检测法和胶原结合检测法分别确认 CB-bFGF 的细胞增殖活性和胶原结合活性。通过荧光成像测量 CB-bFGF 在胶原片（CS）中的保留率。采用大鼠水平牙槽骨缺损模型研究胶原粉末（CP）中 CB-bFGF 的功效。术后 4 周和 8 周，通过微计算机断层扫描、组织学和免疫组织化学分析评估再生效果。
CB-bFGF 具有与 bFGF 相当的增殖活性和胶原结合活性。CB-bFGF 在 CS 中的保留时间长于 bFGF。术后 8 周，与其他组相比，CB-bFGF/CP 组的骨体积、骨矿物质含量和新骨面积显著增加。此外，上皮下生长在 CB-bFGF/CP 组中得到了显著抑制。术后 4 周，CB-bFGF/CP 组再生部位的骨钙素、增殖细胞核抗原和骨桥蛋白阳性细胞数量多于其他组。
CB-bFGF/CP 可有效促进水平骨缺损的骨再生，可能是通过 bFGF 的持续释放。显示了 CB-bFGF 复合材料在垂直轴上改善牙周再生的潜力。
