Ramos Roney S, Oliveira Milena L, Izaguirry Aryele P, Vargas Laura M, Soares Melina B, Mesquita Fernando S, Santos Francielli W, Binelli Mario
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, 13635-900, Brazil.
Laboratory of Reproductive Biotechnology (Biotech), Federal University of Pampa, Uruguaiana, Brazil.
Reprod Biol Endocrinol. 2015 May 10;13:39. doi: 10.1186/s12958-015-0036-x.
In cattle, recent studies have shown positive associations between pre-ovulatory concentrations of estradiol (E2), progesterone (P4) at early diestrus and fertility. However, information on cellular and molecular mechanisms through which sex steroids regulate uterine function to support early pregnancy is lacking. Based on endometrial transcriptome data, objective was to compare function of the redox system in the bovine uterus in response to different periovulatory endocrine milieus.
We employed an animal model to control growth of the pre-ovulatory follicle and subsequent corpus luteum (CL). The large follicle-large CL group (LF-LCL, N=42) presented greater levels of E2 on the day of GnRH treatment (D0; 2.94 vs. 1.27 pg/mL; P=0.0007) and P4 at slaughter on D7 (3.71 vs. 2.62 ng/mL, P=0.01), compared with the small follicle-small CL group (SF-SCL, N=41). Endometrium and uterine washings (N=9, per group) were collected for analyses of variables associated with the uterine redox system.
The SF-SCL group had lower endometrial catalase (0.5 vs. 0.79 U/mg protein, P<0.001) and glutathione peroxidase (GPx; 2.0 vs. 2.43 nmol β-nicotinamide adenine dinucleotide phosphate reduced/min/mg protein, P=0.04) activity, as well as higher lipid peroxidation (28.5 vs. 17.43 nmol malondialdehyde/mg of protein, P<0.001) and superoxide dismutase (SOD) activity (44.77 vs. 37.76 U; P=0.04). There were no differences in the endometrial reactive species (RS) or glutathione (GSH) concentrations between the groups. The uterine washing samples showed no differences in the concentrations of RS or GSH or in total SOD activity (P>0.1). Additionally, catalase, GPx4, SOD1 and SOD2 gene expression was lower in the SF-SCL group than in the LF-LCL group.
We concluded that the intrauterine environment of cows from the LF-LCL group exhibited higher antioxidant activity than that of the cows from the SF-SCL group. We speculate that uterine receptivity and fertility are associated with an optimal redox environment, such as that present in the animals in the LF-LCL group.
在牛身上，最近的研究表明，排卵前雌二醇（E2）浓度、发情前期孕酮（P4）浓度与生育力之间存在正相关。然而，关于性类固醇调节子宫功能以支持早期妊娠的细胞和分子机制的信息尚缺。基于子宫内膜转录组数据，目的是比较牛子宫中氧化还原系统在不同排卵前后内分泌环境下的功能。
我们采用动物模型来控制排卵前卵泡和随后黄体（CL）的生长。与小卵泡 - 小黄体组（SF - SCL，N = 41）相比，大卵泡 - 大黄体组（LF - LCL，N = 42）在促性腺激素释放激素（GnRH）治疗当天（第0天；2.94 vs. 1.27 pg/mL；P = 0.0007）的E2水平更高，在第7天屠宰时的P4水平更高（3.71 vs. 2.62 ng/mL，P = 0.01）。收集子宫内膜和子宫冲洗液（每组N = 9），用于分析与子宫氧化还原系统相关的变量。
SF - SCL组的子宫内膜过氧化氢酶活性较低（0.5 vs. 0.79 U/mg蛋白质，P < 0.001），谷胱甘肽过氧化物酶（GPx）活性较低（2.0 vs. 2.43 nmol还原型β - 烟酰胺腺嘌呤二核苷酸磷酸/分钟/毫克蛋白质，P = 0.04），脂质过氧化水平较高（28.5 vs. 17.43 nmol丙二醛/毫克蛋白质，P < 0.001），超氧化物歧化酶（SOD）活性较高（44.77 vs. 37.76 U；P = 0.04）。两组之间的子宫内膜活性氧（RS）或谷胱甘肽（GSH）浓度没有差异。子宫冲洗液样本在RS或GSH浓度以及总SOD活性方面没有差异（P > 0.1）。此外，SF - SCL组中过氧化氢酶、GPx4、SOD1和SOD2基因表达低于LF - LCL组。
我们得出结论，LF - LCL组奶牛的子宫内环境比SF - SCL组奶牛表现出更高的抗氧化活性。我们推测子宫接受性和生育力与最佳氧化还原环境有关，例如LF - LCL组动物中存在的环境。
