Contreras-Solís Ignacio, Pasciu Valeria, Porcu Cristian, Sotgiu Francesca D, Todorova Neda, Baralla Elena, Mara Laura, Gallus Marilia, Cabiddu Andrea, Dattena Maria, Abecia José Alfonso, Berlinguer Fiammetta
Department of Veterinary Medicine, University of Sassari, 07100 Sassari, Italy.
Department of Animal Science, AGRIS Sardegna, Loc. Bonassai, 07100 Sassari, Italy.
Animals (Basel). 2023 Aug 9;13(16):2565. doi: 10.3390/ani13162565.
This study analyzed the effects of dietary supplementation with by-pass linseed oil (LO; rich in α-linolenic acid) on maternal antioxidant systems at Days 14 and 16 of pregnancy in Sarda ewes. This trial used sixteen dry ewes. Eight ewes (CT group) were fed with a control diet without LO, and eight ewes (LO group) were fed with a diet supplemented with LO (10.8 g of α-linolenic acid/ewe/day). Both diets had similar crude protein and energy levels. The experiment included 10 days of an adaptation period and 31 days of a supplementation period. This supplementation period was divided into Period -2 (from Day -15 to -8), Period -1 (from Day -7 to -1; before synchronized mating period/Day 0), Period +1 (from Day +1 to + 7 after mating), and Period +2 (from Day +8 to +15 after mating). Estrous synchronization was induced in all the ewes using an intravaginal sponge (45 mg fluorgestone acetate) for 14 days and equine chorionic gonadotropin (350 UI/ewe) at the end of the treatment. On Days 14 (CT, N = 4; LO, N = 4) and 16 (CT, N = 4; LO, N = 4) after mating, the ewes were slaughtered. Samples of plasma, uterine, and luteal tissues were collected. Thiols, total antioxidant activity (TEAC), superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content were measured. On Day 16, thiol and TEAC in luteal tissues were higher in the LO group when compared with the control one ( < 0.05). Moreover, TEAC was higher for the LO group in uterine tissues on Days 14 and 16 ( < 0.05). SOD activity was higher in the LO group in luteal and uterine tissues on Day 14 and Day 16, respectively ( < 0.001). On Day 16, uterine MDA content was lower for the LO group ( < 0.001). No differences were found between groups at the plasmatic level. However, the by-pass LO supplementation enhanced the analyzed antioxidant parameters in luteal and uterine tissues. In conclusion, these results demonstrate that by-pass LO supplementation exerted a positive effect on antioxidative defenses on maternal structures during the embryo-maternal recognition period in ewes. Thus, this could contribute to improving the maternal environment during the embryo-maternal recognition period in mammals.
本研究分析了在妊娠第14天和第16天，对撒丁岛母羊补充过瘤胃亚麻籽油（富含α-亚麻酸）对母体抗氧化系统的影响。本试验使用了16只空怀母羊。8只母羊（CT组）饲喂不含亚麻籽油的对照日粮，8只母羊（LO组）饲喂添加了亚麻籽油（10.8克α-亚麻酸/母羊/天）的日粮。两种日粮的粗蛋白和能量水平相似。试验包括10天的适应期和31天的补充期。该补充期分为时期-2（从第-15天至-8天）、时期-1（从第-7天至-1天；在同期发情配种期/第0天之前）、时期+1（配种后第+1天至+7天）和时期+2（配种后第+8天至+15天）。对所有母羊使用阴道海绵栓（45毫克醋酸氟孕酮）诱导发情同步化14天，并在处理结束时注射马绒毛膜促性腺激素（350国际单位/母羊）。在配种后的第14天（CT组，N = 4；LO组，N = 4）和第16天（CT组，N = 4；LO组，N = 4），宰杀母羊。采集血浆、子宫和黄体组织样本。测定硫醇、总抗氧化活性（TEAC）、超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。在第16天，与对照组相比，LO组黄体组织中的硫醇和TEAC含量更高（P<0.05）。此外，在第14天和第16天，LO组子宫组织中的TEAC含量更高（P<0.05）。在第14天和第16天，LO组黄体和子宫组织中的SOD活性分别更高（P<0.001）。在第16天，LO组子宫中的MDA含量更低（P<0.001）。在血浆水平上，两组之间未发现差异。然而，补充过瘤胃亚麻籽油提高了黄体和子宫组织中所分析的抗氧化参数。总之，这些结果表明，在母羊的胚胎-母体识别期，补充过瘤胃亚麻籽油对母体结构的抗氧化防御发挥了积极作用。因此，这可能有助于改善哺乳动物胚胎-母体识别期的母体环境。
