• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

类固醇对输卵管 microRNAs 的调节与牛的 microRNA 加工有关。

Steroidal Regulation of Oviductal microRNAs Is Associated with microRNA-Processing in Beef Cows.

机构信息

North Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Marianna, FL 32446, USA.

Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, 225, Avenida Duque de Caxias, Norte, Jardim, Elite, Pirassununga, SP 13635-900, Brazil.

出版信息

Int J Mol Sci. 2021 Jan 19;22(2):953. doi: 10.3390/ijms22020953.

DOI:10.3390/ijms22020953
PMID:33477993
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7835783/
Abstract

Information on molecular mechanisms through which sex-steroids regulate oviductal function to support early embryo development is lacking. Here, we hypothesized that the periovulatory endocrine milieu affects the miRNA processing machinery and miRNA expression in bovine oviductal tissues. Growth of the preovulatory follicle was controlled to obtain cows that ovulated a small follicle (SF) and subsequently bore a small corpus luteum (CL; SF-SCL) or a large follicle (LF) and large CL (LF-LCL). These groups differed in the periovulatory plasmatic sex-steroid's concentrations. Ampulla and isthmus samples were collected on day four of the estrous cycle. Abundance of DROSHA, DICER1, and AGO4 transcripts was greater in the ampulla than the isthmus. In the ampulla, transcription of these genes was greater for the SF-SCL group, while the opposite was observed in the isthmus. The expression of the 88 most abundant miRNAs and 14 miRNAs in the ampulla and 34 miRNAs in isthmus were differentially expressed between LF-LCL and SF-SCL groups. Integration of transcriptomic and miRNA data and molecular pathways enrichment showed that important pathways were inhibited in the SF-SCL group due to miRNA control. In conclusion, the endocrine milieu affects the miRNA expression in the bovine oviduct in a region-specific manner.

摘要

关于性激素调节输卵管功能以支持早期胚胎发育的分子机制的信息尚不清楚。在这里，我们假设排卵前的内分泌环境会影响 miRNA 加工机制和牛输卵管组织中的 miRNA 表达。通过控制卵泡的生长，获得了排卵小卵泡 (SF) 并随后产生小黄体 (CL; SF-SCL) 或大卵泡 (LF) 和大黄体 (LF-LCL) 的奶牛。这些组在排卵前血浆性激素浓度上存在差异。在发情周期的第四天收集输卵管的壶腹部和峡部样本。DROSHA、DICER1 和 AGO4 转录物的丰度在壶腹部比峡部更高。在壶腹部，SF-SCL 组这些基因的转录更高，而在峡部则相反。在输卵管中，88 种最丰富的 miRNA 和 14 种 miRNA 的表达以及峡部的 34 种 miRNA 在 LF-LCL 和 SF-SCL 组之间存在差异。转录组和 miRNA 数据的整合以及分子通路富集表明，由于 miRNA 的控制，SF-SCL 组的重要通路受到抑制。总之，内分泌环境以特定于区域的方式影响牛输卵管中的 miRNA 表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd3f/7835783/17a6c52f7a1b/ijms-22-00953-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd3f/7835783/9095f928bd42/ijms-22-00953-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd3f/7835783/a908c15e7570/ijms-22-00953-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd3f/7835783/38dd93b1d363/ijms-22-00953-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd3f/7835783/674b2986a22c/ijms-22-00953-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd3f/7835783/c2ab9c873aa0/ijms-22-00953-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd3f/7835783/3a09c9b0ba37/ijms-22-00953-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd3f/7835783/17a6c52f7a1b/ijms-22-00953-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd3f/7835783/9095f928bd42/ijms-22-00953-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd3f/7835783/a908c15e7570/ijms-22-00953-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd3f/7835783/38dd93b1d363/ijms-22-00953-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd3f/7835783/674b2986a22c/ijms-22-00953-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd3f/7835783/c2ab9c873aa0/ijms-22-00953-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd3f/7835783/3a09c9b0ba37/ijms-22-00953-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd3f/7835783/17a6c52f7a1b/ijms-22-00953-g007.jpg

相似文献

1
Steroidal Regulation of Oviductal microRNAs Is Associated with microRNA-Processing in Beef Cows.类固醇对输卵管 microRNAs 的调节与牛的 microRNA 加工有关。
Int J Mol Sci. 2021 Jan 19;22(2):953. doi: 10.3390/ijms22020953.
2
Local sex steroid hormone milieu in the bovine oviduct ipsilateral and contralateral to preovulatory follicle or corpus luteum during the periovulatory phase.在排卵周期中，牛排卵前卵泡或黄体同侧和对侧输卵管局部性激素环境。
Domest Anim Endocrinol. 2021 Jan;74:106515. doi: 10.1016/j.domaniend.2020.106515. Epub 2020 Jun 26.
3
Size of the Ovulatory Follicle Dictates Spatial Differences in the Oviductal Transcriptome in Cattle.排卵卵泡大小决定牛输卵管转录组的空间差异。
PLoS One. 2015 Dec 23;10(12):e0145321. doi: 10.1371/journal.pone.0145321. eCollection 2015.
4
Sex steroids modulate morphological and functional features of the bovine oviduct.性激素调节牛输卵管的形态和功能特征。
Cell Tissue Res. 2017 Nov;370(2):319-333. doi: 10.1007/s00441-017-2666-0. Epub 2017 Aug 2.
5
Sex steroids drive the remodeling of oviductal extracellular matrix in cattle.性激素驱动牛输卵管细胞外基质的重塑。
Biol Reprod. 2018 Sep 1;99(3):590-599. doi: 10.1093/biolre/ioy083.
6
Modulation of periovulatory endocrine profiles in beef cows: consequences for endometrial glucose transporters and uterine fluid glucose levels.肉牛母牛排卵前后内分泌特征的调节：对子宫内膜葡萄糖转运蛋白和子宫液葡萄糖水平的影响。
Domest Anim Endocrinol. 2015 Jan;50:83-90. doi: 10.1016/j.domaniend.2014.09.005. Epub 2014 Sep 28.
7
Manipulation of the periovulatory sex steroidal milieu affects endometrial but not luteal gene expression in early diestrus Nelore cows.在早期发情期，操纵排卵前后的性类固醇环境会影响内米罗牛的子宫内膜但不影响黄体基因表达。
Theriogenology. 2014 Apr 1;81(6):861-9. doi: 10.1016/j.theriogenology.2013.12.022. Epub 2013 Dec 27.
8
The periovulatory endocrine milieu affects the uterine redox environment in beef cows.排卵前后的内分泌环境会影响肉牛的子宫氧化还原环境。
Reprod Biol Endocrinol. 2015 May 10;13:39. doi: 10.1186/s12958-015-0036-x.
9
Oviductal transcriptional profiling of a bovine fertility model by next-generation sequencing.利用下一代测序技术对牛生育力模型进行输卵管转录谱分析。
Genom Data. 2017 Jun 15;13:27-29. doi: 10.1016/j.gdata.2017.06.004. eCollection 2017 Sep.
10
Regulation of bovine oviductal NO synthesis by follicular steroids and prostaglandins.卵泡类固醇和前列腺素对牛输卵管一氧化氮合成的调节
Reproduction. 2016 Jun;151(6):577-87. doi: 10.1530/REP-15-0254. Epub 2016 Mar 3.

引用本文的文献

1
Oocyte Maturation and miRNAs: Studying a Complicate Interaction to Reveal Possible Biomarkers for Female Infertility.卵母细胞成熟与微小RNA：研究复杂相互作用以揭示女性不孕症的潜在生物标志物
Diseases. 2024 Jun 4;12(6):121. doi: 10.3390/diseases12060121.
2
Corpus luteum presence in the bovine ovary increase intrafollicular progesterone concentration: consequences in follicular cells gene expression and follicular fluid small extracellular vesicles miRNA contents.牛卵巢黄体的存在增加了卵泡内孕酮的浓度：对卵泡细胞基因表达和卵泡液小细胞外囊泡 miRNA 含量的影响。
J Ovarian Res. 2024 Mar 18;17(1):65. doi: 10.1186/s13048-024-01387-3.
3

本文引用的文献

1
Mitochondria-targeted therapy rescues development and quality of embryos derived from oocytes matured under oxidative stress conditions: a bovine in vitro model.线粒体靶向治疗可挽救氧化应激条件下成熟卵母细胞来源胚胎的发育和质量：牛体外模型。
Hum Reprod. 2019 Oct 2;34(10):1984-1998. doi: 10.1093/humrep/dez161.
2
Role of endoplasmic reticulum stress on developmental competency and cryo-tolerance in bovine embryos.内质网应激对牛胚胎发育能力和抗冷冻能力的作用。
Theriogenology. 2020 Jan 15;142:131-137. doi: 10.1016/j.theriogenology.2019.09.042. Epub 2019 Sep 28.
3
Murine Oviductosomes (OVS) microRNA profiling during the estrous cycle: Delivery of OVS-borne microRNAs to sperm where miR-34c-5p localizes at the centrosome.
OncoUroMiR: Circulating miRNAs for Detection and Discrimination of the Main Urological Cancers Using a ddPCR-Based Approach.
OncoUroMiR：一种基于 ddPCR 的方法，用于检测和区分主要泌尿系统癌症的循环 miRNA。
Int J Mol Sci. 2023 Sep 9;24(18):13890. doi: 10.3390/ijms241813890.
4
An interactive analysis of the mouse oviductal miRNA profiles.小鼠输卵管微小RNA谱的交互式分析。
Front Cell Dev Biol. 2022 Oct 19;10:1015360. doi: 10.3389/fcell.2022.1015360. eCollection 2022.
5
Evaluation of circulating extracellular vesicles and miRNA in neutered and obese female dogs.绝育肥胖雌性犬循环细胞外囊泡和微小RNA的评估
Sci Rep. 2022 Sep 30;12(1):16439. doi: 10.1038/s41598-022-20523-x.
6
Changes in Oviductal Cells and Small Extracellular Vesicles miRNAs in Pregnant Cows.怀孕母牛输卵管细胞和小细胞外囊泡微小RNA的变化
Front Vet Sci. 2021 Mar 4;8:639752. doi: 10.3389/fvets.2021.639752. eCollection 2021.
小鼠输卵管小体（OVS）在动情周期中的 microRNA 谱分析：OVS 携带的 microRNAs 递送至精子，miR-34c-5p 定位于中心体。
Sci Rep. 2018 Oct 31;8(1):16094. doi: 10.1038/s41598-018-34409-4.
4
Deciphering the oviductal extracellular vesicles content across the estrous cycle: implications for the gametes-oviduct interactions and the environment of the potential embryo.解析发情周期中输卵管细胞外囊泡的内容物：对配子-输卵管相互作用和潜在胚胎环境的影响。
BMC Genomics. 2018 Aug 22;19(1):622. doi: 10.1186/s12864-018-4982-5.
5
Sex steroids drive the remodeling of oviductal extracellular matrix in cattle.性激素驱动牛输卵管细胞外基质的重塑。
Biol Reprod. 2018 Sep 1;99(3):590-599. doi: 10.1093/biolre/ioy083.
6
Metazoan MicroRNAs.后生动物 MicroRNAs。
Cell. 2018 Mar 22;173(1):20-51. doi: 10.1016/j.cell.2018.03.006.
7
CEMiTool: a Bioconductor package for performing comprehensive modular co-expression analyses.CEMiTool：一个用于执行全面模块化共表达分析的 Bioconductor 软件包。
BMC Bioinformatics. 2018 Feb 20;19(1):56. doi: 10.1186/s12859-018-2053-1.
8
Sex Steroid-Mediated Control of Oviductal Function in Cattle.性类固醇对牛输卵管功能的调控
Biology (Basel). 2018 Feb 2;7(1):15. doi: 10.3390/biology7010015.
9
, , and microRNA machinery genes are differentially expressed in developing female reproductive organs and overexpressed in cancerous ovaries of chickens.在雌性生殖器官的发育过程中， 和 microRNA 机器基因的表达存在差异，并且在鸡的癌变卵巢中过度表达。
J Anim Sci. 2017 Nov;95(11):4857-4868. doi: 10.2527/jas2017.1846.
10
Sex steroids modulate morphological and functional features of the bovine oviduct.性激素调节牛输卵管的形态和功能特征。
Cell Tissue Res. 2017 Nov;370(2):319-333. doi: 10.1007/s00441-017-2666-0. Epub 2017 Aug 2.