用半纤维素酶补充剂进行生物消减以减少酶水解抑制剂。
Bioabatement with hemicellulase supplementation to reduce enzymatic hydrolysis inhibitors.
机构信息
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150001, China.
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States.
出版信息
Bioresour Technol. 2015 Aug;190:412-5. doi: 10.1016/j.biortech.2015.04.064. Epub 2015 Apr 24.
A stepwise removal of inhibitory compounds by bioabatement combined with hemicellulase supplementation was conducted to enhance cellulose hydrolysis of liquid hot water-pretreated corn stover. Results showed that the fungus Coniochaeta ligniaria NRRL30616 eliminated most of the enzyme and fermentation inhibitors from liquid hot water-pretreated corn stover hydrolysates. Moreover, addition of hemicellulases after bioabatement and before enzymatic hydrolysis of cellulose achieved 20% higher glucose yields compared to non-treated samples. This work presents the mechanisms by which supplementation of the fungus with hemicellulase enzymes enables maximal conversion, and confirms the inhibitory effect of xylo-oligosaccharides in corn stover hydrolysates once the dominant inhibitory effect of phenolic compounds is removed.
采用生物消减与半纤维素酶补料相结合的方式逐步去除抑制物，以提高液体热预处理玉米秸秆的纤维素水解效率。结果表明，真菌康氏木霉 NRRL30616 可去除液体热预处理玉米秸秆水解液中大部分的酶和发酵抑制剂。此外，生物消减处理后添加半纤维素酶，并在纤维素酶解前添加，与未经处理的样品相比，葡萄糖得率提高了 20%。本研究阐述了真菌补充半纤维素酶可实现最大转化的机制，并证实了木二糖等低聚糖在玉米秸秆水解液中的抑制作用，一旦去除了主要的酚类化合物抑制作用。
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