Kang Bo, Xu Qilin, Chen Ziyu, Wu Yongsheng, Yang Su, Yang Xicheng, Zhang Zhao, Jiang Dongmei
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, PR China.
Institute of Animal Science, Chengdu Academy of Agriculture and Forestry Sciences, Chengdu, PR China.
Reprod Biol. 2018 Mar;18(1):60-65. doi: 10.1016/j.repbio.2018.01.003. Epub 2018 Jan 12.
Spermine synthase (SPMS), which converts spermidine into spermine, is essential for normal cell growth and development processes in humans and other mammals, but the molecular characterization and expression profiling of the SPMS gene remain undetermined in goose tissues and ovarian follicles. In this study, the SPMS cDNA sequence of the Sichuan white goose was cloned and analysed, and SPMS mRNA expression was profiled in various tissues and ovarian follicles. The results showed that the open reading frame of the SPMS cDNA sequence was 1092 bp in length, encoding 363 amino acids with a molecular weight of 41 kDa. Among all the examined tissues, SPMS expression was highest in the spleen and cerebrum and lowest in the breast and thigh muscles. SPMS expression in the F1 follicle was significantly higher than that in the POF (except for POF2) (P < 0.05). Our results indicate that SPMS might play an important role in follicular development and ovulation.
精胺合酶（SPMS）可将亚精胺转化为精胺，对人类和其他哺乳动物的正常细胞生长与发育过程至关重要，但SPMS基因在鹅组织和卵巢卵泡中的分子特征及表达谱仍未明确。在本研究中，克隆并分析了四川白鹅的SPMS cDNA序列，并对其在不同组织和卵巢卵泡中的mRNA表达进行了分析。结果显示，SPMS cDNA序列的开放阅读框长度为1092 bp，编码363个氨基酸，分子量为41 kDa。在所有检测组织中，SPMS在脾脏和大脑中的表达最高，在胸肌和大腿肌肉中最低。F1卵泡中的SPMS表达显著高于POF（POF2除外）（P<0.05）。我们的结果表明，SPMS可能在卵泡发育和排卵中发挥重要作用。
