一种RNA伴侣介导的光触发RNA退火
Light-Triggered RNA Annealing by an RNA Chaperone.
作者信息
Panja Subrata, Paul Rakesh, Greenberg Marc M, Woodson Sarah A
机构信息
T.C. Jenkins Department of Biophysics, Johns Hopkins University, 3400 N. Charles St., Baltimore MD 21218 (USA).
Department of Chemistry, Johns Hopkins University, 3400 N. Charles St., Baltimore MD 21218 (USA).
出版信息
Angew Chem Int Ed Engl. 2015 Jun 15;54(25):7281-4. doi: 10.1002/anie.201501658. Epub 2015 May 8.
Abstract
Non-coding antisense RNAs regulate bacterial genes in response to nutrition or environmental stress, and can be engineered for artificial gene control. The RNA chaperone Hfq accelerates antisense pairing between non-coding RNAs and their mRNA targets, by a mechanism still unknown. We used a photocaged guanosine derivative in an RNA oligonucleotide to temporally control Hfq catalyzed annealing. Using a fluorescent molecular beacon as a reporter, we observed RNA duplex formation within 15 s following irradiation (3 s) of photocaged RNA complexed with Hfq. The results showed that the Hfq chaperone directly stabilizes the initiation of RNA base pairs, and suggests a strategy for light-activated control of gene expression by non-coding RNAs.
摘要
非编码反义RNA可响应营养或环境压力调节细菌基因，并且可被设计用于人工基因控制。RNA伴侣蛋白Hfq通过一种尚不清楚的机制加速非编码RNA与其mRNA靶标之间的反义配对。我们在RNA寡核苷酸中使用了一种光笼化鸟苷衍生物来临时控制Hfq催化的退火。使用荧光分子信标作为报告分子，我们观察到与Hfq复合的光笼化RNA在照射（3秒）后15秒内形成了RNA双链体。结果表明，Hfq伴侣蛋白直接稳定了RNA碱基对的起始，并提出了一种通过非编码RNA进行光激活基因表达控制的策略。
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