Thomas C. Jenkins Department of Biophysics, Johns Hopkins University, 3400 N. Charles St.,5, Baltimore, MD, 21218, USA.
Laboratory of Single-Molecule Biophysics, International Institute of Molecular and Cell Biology in Warsaw, Trojdena 4, Warsaw, 02-109, Poland.
Nat Commun. 2024 Mar 7;15(1):2069. doi: 10.1038/s41467-024-46316-6.
RNA-guided enzymes must quickly search a vast sequence space for their targets. This search is aided by chaperones such as Hfq, a protein that mediates regulation by bacterial small RNAs (sRNAs). How RNA binding proteins enhance this search is little known. Using single-molecule Förster resonance energy transfer, we show that E. coli Hfq performs a one-dimensional scan in which compaction of the target RNA delivers sRNAs to sites distant from the location of Hfq recruitment. We also show that Hfq can transfer an sRNA between different target sites in a single mRNA, favoring the most stable duplex. We propose that compaction and segmental transfer, combined with repeated cycles of base pairing, enable the kinetic selection of optimal sRNA targets. Finally, we show that RNA compaction and sRNA transfer require conserved arginine patches. We suggest that arginine patches are a widespread strategy for enabling the movement of RNA across protein surfaces.
RNA 指导的酶必须快速搜索其靶标广阔的序列空间。这种搜索得到了 Hfq 等伴侣蛋白的帮助，Hfq 是一种介导细菌小 RNA（sRNA）调控的蛋白质。RNA 结合蛋白如何增强这种搜索尚不清楚。我们使用单分子Förster 共振能量转移技术表明，大肠杆菌 Hfq 进行一维扫描，其中靶 RNA 的紧缩将 sRNA 递送到远离 Hfq 招募位置的位点。我们还表明，Hfq 可以在单个 mRNA 中的不同靶标之间转移 sRNA，优先选择最稳定的双链体。我们提出，紧缩和片段转移，加上反复的碱基配对循环，使最佳 sRNA 靶标的动力学选择成为可能。最后，我们表明 RNA 紧缩和 sRNA 转移需要保守的精氨酸斑。我们认为精氨酸斑是一种广泛的策略，可使 RNA 在蛋白质表面上移动。
Zotero 插件
