Filice M, Romero O, Gutiérrez-Fernández J, de Las Rivas B, Hermoso J A, Palomo J M
Departamento de Biocatálisis, Instituto de Catálisis, CSIC, Marie Curie 2, Campus UAM, 28049, Madrid, Spain.
Chem Commun (Camb). 2015 Jun 7;51(45):9324-7. doi: 10.1039/c5cc02450a.
A solid-phase strategy using lipase as a biomolecular scaffold to produce a large amount of Cu(2+)-metalloenzyme is proposed here. The application of this protocol on different 3D cavities of the enzyme allows creating a heterogeneous artificial metallolipase showing chimeric catalytic activity. The artificial catalyst was assessed in Diels-Alder cycloaddition reactions and cascade reactions showing excellent catalytic properties.
本文提出了一种利用脂肪酶作为生物分子支架来大量生产Cu(2+)金属酶的固相策略。该方案应用于酶的不同三维空腔，能够构建出具有嵌合催化活性的多相人工金属脂肪酶。该人工催化剂在狄尔斯-阿尔德环加成反应和串联反应中进行了评估，显示出优异的催化性能。
