Fang Yifeng, Liang Xiao, Jiang Wenbin, Li Jianbo, Xu Junfen, Cai Xiujun
The Second Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310016, China.
Department of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China.
PLoS One. 2015 May 11;10(5):e0126875. doi: 10.1371/journal.pone.0126875. eCollection 2015.
Cyclin B1, a mitotic cyclin, has been implicated in malignances. However, its contribution to colorectal cancer invasion and metastasis are still not well understood. Here, we demonstrated that the invasion and metastasis of colorectal cancer is regulated by Cyclin B1. Overexpression of Cyclin B1 was observed in colorectal cancer tissues, but this elevated expression was negatively associated with lymph node metastasis, distant metastasis stage, and TNM stage. The Kaplan-Meier survival analysis proved that low Cyclin B1 expression was associated with poor overall survival of patients with colorectal cancer. Inhibition of Cyclin B1 in colorectal cancer cells enhanced the cell migration and invasion of three different colorectal cancer cell lines. In studying the possible mechanism by which Cyclin B1 suppresses colorectal cancer invasion and metastasis, we observed that suppression of Cyclin B1 decreased the expression of E-cadherin protein level. Our findings suggest that Cyclin B1 could suppress the invasion and metastasis of colorectal cancer cells through regulating E-cadherin expression, which enables the development of potential intervention strategies for colorectal cancer.
细胞周期蛋白B1是一种有丝分裂细胞周期蛋白，与恶性肿瘤有关。然而，其在结直肠癌侵袭和转移中的作用仍未完全明确。在此，我们证明了结直肠癌的侵袭和转移受细胞周期蛋白B1调控。在结直肠癌组织中观察到细胞周期蛋白B1的过表达，但这种表达升高与淋巴结转移、远处转移分期和TNM分期呈负相关。Kaplan-Meier生存分析证明，细胞周期蛋白B1低表达与结直肠癌患者的总体生存率低有关。抑制结直肠癌细胞中的细胞周期蛋白B1可增强三种不同结直肠癌细胞系的细胞迁移和侵袭能力。在研究细胞周期蛋白B1抑制结直肠癌侵袭和转移的可能机制时，我们观察到细胞周期蛋白B1的抑制降低了E-钙黏蛋白的蛋白水平表达。我们的研究结果表明，细胞周期蛋白B1可通过调节E-钙黏蛋白的表达来抑制结直肠癌细胞的侵袭和转移，这为结直肠癌潜在干预策略的开发提供了可能。
