Lima Francisco Esmaile de Sales, Cibulski Samuel Paulo, Elesbao Felipe, Carnieli Junior Pedro, Batista Helena Beatriz de Carvalho Ruthner, Roehe Paulo Michel, Franco Ana Cláudia
Veterinary Research Institute "Desiderio Finamor" (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS, CEP 92990-000, Brazil,
Virus Genes. 2013 Oct;47(2):378-81. doi: 10.1007/s11262-013-0947-6. Epub 2013 Jul 5.
This paper describes the first detection of adenovirus in a Brazilian Desmodus rotundus bat, the common vampire bat. As part of a continuous rabies surveillance program, three bat specimens were captured in Southern Brazil. Total DNA was extracted from pooled organs and submitted to a nested PCR designed to amplify a 280 bp long portion of the DNA polymerase gene of adenoviruses. One positive sample was subjected to nucleotide sequencing, confirming that this DNA fragment belongs to a member of the genus Mastadenovirus. This sequence is approximately 25 % divergent at the nucleotide level from equine adenovirus 1 and two other recently characterized bat adenoviruses.
本文描述了在巴西普通吸血蝙蝠——圆叶叶口蝠中首次检测到腺病毒。作为一项持续的狂犬病监测计划的一部分，在巴西南部捕获了三只蝙蝠标本。从合并的器官中提取总DNA，并进行巢式PCR，以扩增腺病毒DNA聚合酶基因的一段280bp长的片段。对一个阳性样本进行核苷酸测序，证实该DNA片段属于 Mastadenovirus 属的一个成员。该序列在核苷酸水平上与马腺病毒1和其他两种最近鉴定的蝙蝠腺病毒的差异约为25%。
