Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China.
Department of Respiratory Disease, Xi'an Central Hospital, Xi'an 710003, China.
Carbohydr Polym. 2015;127:215-21. doi: 10.1016/j.carbpol.2015.03.070. Epub 2015 Mar 30.
In this study, one polysaccharide (GFP1), with an average molecular weight of 1.4 × 10(5)Da, was isolated from Ginseng fruits. GFP1 was composed of galactose, glucose, rhamnose, and arabinose in a molar ratio of 6.1:2.0:1.1:3.2, and had a backbone mainly consisting of (1 → 6)-linked-Galp, (1 → 3,6)-linked-Galp and (1 → 3,6)-linked-Glcp residues, which was terminated with terminal (1 →)-linked-Araf or -Rhap attached to O-3 position of (1 → 3,6)-linked-Galp and (1 → 3,6)-linked-Glcp. We also evaluated the effect of GFP1 on anti-tumor immune response in Lewis lung carcinoma (LLC)-bearing mouse model and explored the possible mechanism. GPF1 could significantly inhibit tumor growth and lung metastasis in vivo, increase the relative spleen and thymus weight, promote ConA or LPS-induced spleen lymphocytes proliferation, elevate the activities of NK cell in spleen, and increase the serum concentration of interleukin-2 (IL-2) and interferon-γ (IFN-γ), as well as the ratio of CD4(+)/CD8(+) in LLC-bearing mice. All these findings implied that GFP1 could effectively inhibit tumor growth and lung metastasis via activating immune function and provide insights into the mechanism of GFP1 in the prevention and treatment of lung cancer.
在这项研究中，从人参果实中分离得到一种多糖（GFP1），其平均分子量为 1.4×10(5)Da。GFP1 由半乳糖、葡萄糖、鼠李糖和阿拉伯糖以摩尔比 6.1:2.0:1.1:3.2 组成，其主链主要由（1→6）-连接的 Galp、（1→3,6）-连接的 Galp 和（1→3,6）-连接的 Glcp 残基组成，末端由连接到（1→3,6）-连接的 Galp 和（1→3,6）-连接的 Glcp 的 O-3 位的末端（1→）-连接的 Ara f 或 Rhap 终止。我们还评估了 GFP1 在 Lewis 肺癌（LLC）荷瘤小鼠模型中对抗肿瘤免疫反应的影响，并探讨了可能的机制。GFP1 可显著抑制体内肿瘤生长和肺转移，增加脾脏和胸腺相对重量，促进 ConA 或 LPS 诱导的脾淋巴细胞增殖，提高脾 NK 细胞活性，增加血清白细胞介素-2（IL-2）和干扰素-γ（IFN-γ）浓度，并增加 LLC 荷瘤小鼠中 CD4(+)/CD8(+)的比例。所有这些发现表明，GFP1 通过激活免疫功能可有效抑制肿瘤生长和肺转移，并为 GFP1 预防和治疗肺癌的机制提供了新的见解。
