Gao Ying, Xu Dan, Zhao Lei, Zhang Min, Sun Yeqing
Institute of Environmental Systems Biology, College of Environmental Science and Engineering, Dalian Maritime University , Dalian, Liaoning , P. R. China.
Int J Radiat Biol. 2015 Jul;91(7):531-9. doi: 10.3109/09553002.2015.1043754. Epub 2015 May 12.
Space radiations and microgravity both could cause DNA damage in cells, but the effects of microgravity on DNA damage response to space radiations are still controversial.
A mRNA microarray and microRNA micro- array in dauer larvae of Caenorhabditis elegans (C. elegans) that endured spaceflight environment and space radiations environment during 16.5-day Shenzhou-8 space mission was performed.
Twice as many transcripts significantly altered in the spaceflight environment than space radiations alone. The majority of alterations were related to protein amino acid dephosphorylation and histidine metabolic and catabolic processes. From about 900 genes related to DNA damage response, 38 differentially expressed genes were extracted; most of them differentially expressed under spaceflight environment but not space radiations, although the identical directions of alteration were observed in both cases. cel-miR-81, cel- miR-82, cel-miR-124 and cel-miR-795 were predicted to regulate DNA damage response through four different anti-correlated genes.
Evidence was provided that, in the presence of space radiations, microgravity probably enhanced the DNA damage response in C. elegans by integrating the transcriptome and microRNome.
空间辐射和微重力均可导致细胞中的DNA损伤，但微重力对DNA损伤响应空间辐射的影响仍存在争议。
对在神舟八号16.5天太空任务期间经受太空飞行环境和太空辐射环境的秀丽隐杆线虫（C. elegans） dauer幼虫进行mRNA微阵列和微小RNA微阵列分析。
在太空飞行环境中显著改变的转录本数量是仅受太空辐射环境影响的两倍。大多数改变与蛋白质氨基酸去磷酸化以及组氨酸代谢和分解代谢过程有关。从约900个与DNA损伤反应相关的基因中，提取出38个差异表达基因；其中大多数在太空飞行环境下差异表达，而在太空辐射环境下未差异表达，尽管在两种情况下观察到的改变方向相同。预测cel-miR-81、cel-miR-82、cel-miR-124和cel-miR-795通过四个不同的反相关基因调节DNA损伤反应。
有证据表明，在存在空间辐射的情况下，微重力可能通过整合转录组和微小RNA组增强秀丽隐杆线虫中的DNA损伤反应。
