Yun Y-E, Yu J-N, Nam G H, Ryu S-A, Kim S, Oh K, Lim C E
National Institute of Biological Resources, Environmental Research Complex, Incheon, Korea.
National Institute of Biological Resources, Environmental Research Complex, Incheon, Korea
Genet Mol Res. 2015 May 11;14(2):4812-7. doi: 10.4238/2015.May.11.13.
We used next-generation sequencing to develop 9 novel microsatellite markers in Aconitum austrokoreense, an endemic and endangered medicinal plant in Korea. Owing to its very limited distribution, over-harvesting for traditional medicinal purposes, and habitat loss, the natural populations are dramatically declining in Korea. All novel microsatellite markers were successfully genotyped using 64 samples from two populations (Mt. Choejeong, Gyeongsangbuk-do and Ungseokbong, Gyeongsangnam-do) of Gyeongsang Province. The number of alleles ranged from 2 to 7 per locus in each population. Observed and expected heterozygosities ranged from 0.031 to 0.938 and from 0.031 to 0.697, respectively. The novel markers will be valuable tools for assessing the genetic diversity of A. austrokoreense and for germplasm conservation of this endangered species.
我们利用下一代测序技术，在韩国特有的濒危药用植物朝鲜乌头（Aconitum austrokoreense）中开发了9个新的微卫星标记。由于其分布极为有限、因传统药用目的过度采挖以及栖息地丧失，韩国的自然种群数量正在急剧下降。使用庆尚省两个种群（庆尚北道草亭山和庆尚南道云石山）的64个样本，成功对所有新的微卫星标记进行了基因分型。每个种群中每个位点的等位基因数量从2到7不等。观察到的杂合度和预期杂合度分别在0.031至0.938和0.031至0.697之间。这些新标记将成为评估朝鲜乌头遗传多样性以及保护这种濒危物种种质资源的有价值工具。
