State Key Laboratory of Grassland and Agro-Ecosystems, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China.
Mol Biol Rep. 2020 Jan;47(1):727-729. doi: 10.1007/s11033-019-05160-4. Epub 2019 Nov 1.
Mating plays key roles in the demographic and genetic dynamics of populations. Estimates of mating portfolios and system based on progeny array (PA) method required highly polymorphic genetic markers, of which microsatellite is a good choice. In this study, we reported 19 polymorphic microsatellite loci for Aconitum gymnandrum. The number of alleles per locus ranged from 2 to 12. Observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.219 to 0.842, respectively. Seven loci showed significant deviation from Hardy-Weinberg equilibrium. These markers will provide a useful tool for pollination ecology and population genetic studies of A. gymnandrum in Qinghai-Tibet plateau.
交配在种群的人口和遗传动态中起着关键作用。基于后代数组 (PA) 方法的交配组合和系统的估计需要高度多态的遗传标记，其中微卫星是一个很好的选择。在这项研究中，我们报道了 19 个多态性微卫星位点的 Aconitum gymnandrum。每个位点的等位基因数从 2 到 12 不等。观察到的和预期的杂合度分别从 0.000 到 1.000 和从 0.219 到 0.842 不等。七个位点表现出与哈迪-温伯格平衡的显著偏离。这些标记将为青藏高原 A. gymnandrum 的授粉生态学和种群遗传研究提供有用的工具。
