Kobrinsky Evgeny
National Institute on Aging, National Institutes of Health, 251 Bayview Blvd., Baltimore, MD, 21224, US.
Curr Mol Pharmacol. 2015;8(1):54-60. doi: 10.2174/1874467208666150507093601.
The major function of the voltage-gated calcium channels is to provide the Ca(2+) flux into the cell. L-type voltage-gated calcium channels (Cav1) serve as voltage sensors that couple membrane depolarization to many intracellular processes. Electrical activity in excitable cells affects gene expression through signaling pathways involved in the excitation-transcription (E-T) coupling. E-T coupling starts with activation of the Cav1 channel and results in initiation of the cAMP-response element binding protein (CREB)-dependent transcription. In this review we discuss the new quantitative approaches to measuring E-T signaling events. We describe the use of wavelet transform to detect heterogeneity of transcriptional activation in nuclei. Furthermore, we discuss the properties of discovered microdomains of nuclear signaling associated with the E-T coupling and the basis of the frequency-dependent transcriptional regulation.
电压门控钙通道的主要功能是使Ca(2+)流入细胞。L型电压门控钙通道（Cav1）作为电压传感器，将膜去极化与许多细胞内过程相偶联。可兴奋细胞中的电活动通过参与兴奋-转录（E-T）偶联的信号通路影响基因表达。E-T偶联始于Cav1通道的激活，并导致依赖于环磷酸腺苷反应元件结合蛋白（CREB）的转录起始。在本综述中，我们讨论了测量E-T信号事件的新定量方法。我们描述了使用小波变换检测细胞核中转录激活的异质性。此外，我们讨论了与E-T偶联相关的已发现的核信号微结构域的特性以及频率依赖性转录调控的基础。
