草药制剂异常黑胆质成熟剂的细胞毒性作用研究
Investigations into cytotoxic effects of the herbal preparation Abnormal Savda Munziq.
作者信息
Kühnel Harald, Adilijiang Abulimiti, Dadak Agnes, Wieser Matthias, Upur Halmurat, Stolze Klaus, Grillari Johannes, Strasser Alois
机构信息
Department of Biomedical Sciences, Institute of Physiology, Pathophysiology and Biophysics, University of Veterinary Medicine Vienna, Vienna, A-1210, Austria.
出版信息
Chin J Integr Med. 2015 May 12. doi: 10.1007/s11655-015-2132-3.
OBJECTIVE
To evaluate the effects of Abnormal Savda Munziq (ASMq), a traditional herbal medicine, for the prevention and treatment of human diseases, e.g. bowel cancer.
METHODS
The parameters total polyphenol content, cell proliferation and DNA-damage as well as RNA and protein-oxidation were analysed in vitro. Besides, the expressions of miRNA and tumor suppressor genes as well as cellular senescence were evaluated.
RESULTS
ASMq had a high polyphenol content and induced damage to proteins, RNA as well as to DNA, which is correlated with its cytotoxicity. Furthermore ASMq up-regulated the tumor suppressor genes p21, p53 and p16 and down-regulated the micro-RNAs hsa-mir-17 and hsa-mir-106b. In addition cellular growth arrest and SA-β-gal-staining were induced.
CONCLUSION
ASMq has the ability to induce DNA damage and cellular senescence, which are double-edged mechanisms in fighting cancer, as they might also have harmful side effects.
目的
评估传统草药异常黑胆质成熟剂（ASMq）对人类疾病如肠癌的预防和治疗效果。
方法
在体外分析总多酚含量、细胞增殖、DNA损伤以及RNA和蛋白质氧化等参数。此外，评估了微小RNA（miRNA）和肿瘤抑制基因的表达以及细胞衰老情况。
结果
ASMq含有高含量的多酚，并能诱导蛋白质、RNA以及DNA损伤，这与其细胞毒性相关。此外，ASMq上调肿瘤抑制基因p21、p53和p16，并下调微小RNA hsa-mir-17和hsa-mir-106b。另外，诱导了细胞生长停滞和SA-β-半乳糖苷酶染色。
结论
ASMq具有诱导DNA损伤和细胞衰老的能力，这在抗癌过程中是双刃剑机制，因为它们可能也有有害的副作用。
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