Yang Tao, Aimaiti Mutalifu, Su Deqi, Miao Weiwei, Zhou Bin, Maimaitiyiming Dilinuer, Yusup Abdiryim, Upur Halmurat, Aikemu Ainiwaer
Department of pharmaceutical analysis, Xinjiang Medical University, Urumqi, 830011, China.
Central Laboratory of Xinjiang Medical University, Urumqi, 830011, China.
BMC Complement Altern Med. 2017 Apr 7;17(1):201. doi: 10.1186/s12906-017-1685-4.
Abnormal Savda Munziq (ASMq) is a traditional prescription in Uyghur Medicine, and its treatment of complex diseases such as tumors and asthma has been proven to be effective in Uyghur medical clinical practice. The efficacy-enhancing and toxicity-reducing properties of ASMq were studied on mice with transplanted cervical cancer (U27) tumors, which were treated with 5-fluorouracil (5-FU) in this work.
To investigate the synergistic effect of ASMq and 5-FU on U27 cells, inhibitory effects on cell proliferation were determined through a MTT assay. 48 Kunming mice which were randomly divided in to 6 groups: control group, model group, 5-FU group, 5-FU combine with ASMq low-dose group, 5-FU combine with ASMq medium-dose group, and 5-FU combine with ASMq high- dose group, the inhibition rate of the tumor, the viscera indexes, and the content of serum tumor necrosis factor-α (TNF-α), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. The expression levels of transforming growth factor-β1 (TGF-β1) and human papillomavirus type 16 E2 (HPV16 E2) protein were assessed by Western blot. Pathological changes in the liver were observed.
The inhibition rates of tumors, the 5-FU + ASMq.H group(80.64%), 5-FU + ASMq.M group (90.67%), 5-FU + ASMq.L group (72.03%) and 5-FU group (66.89%), clearly indicated that the effects of tumor inhibition. The thymus index and spleen index were increased, and the serum concentration of TNF-α increased while ALT and AST concentrations were decreased, and TNF-α protein expression were increased while TGF-β1 and HPV16 E2 were decreased. ASMq might can improve livers central vein hyperemia and interstitial edema, and preserve the radial structure of the hepatic cords.
The results suggested that ASMq might reduce toxicity and enhance the efficacy of the chemotherapeutic drug 5-fluorouracil in the treatment of cervical carcinoma.
异常黑胆质成熟剂（ASMq）是维吾尔医学中的一种传统方剂，其在肿瘤和哮喘等复杂疾病治疗中的有效性已在维吾尔医学临床实践中得到证实。本研究以移植宫颈癌（U27）肿瘤的小鼠为对象，研究ASMq与5-氟尿嘧啶（5-FU）联合应用时的增效减毒作用。
为研究ASMq与5-FU对U27细胞的协同作用，通过MTT法测定对细胞增殖的抑制作用。将48只昆明小鼠随机分为6组：对照组、模型组、5-FU组、5-FU联合ASMq低剂量组、5-FU联合ASMq中剂量组、5-FU联合ASMq高剂量组，测定肿瘤抑制率、脏器指数、血清肿瘤坏死因子-α（TNF-α）、丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）含量。通过蛋白质印迹法评估转化生长因子-β1（TGF-β1）和人乳头瘤病毒16型E2（HPV16 E2）蛋白的表达水平。观察肝脏的病理变化。
肿瘤抑制率方面，5-FU + ASMq.H组（80.64%）、5-FU + ASMq.M组（90.67%）、5-FU + ASMq.L组（72.03%）和5-FU组（66.89%），明确显示出肿瘤抑制效果。胸腺指数和脾脏指数升高，血清TNF-α浓度升高，而ALT和AST浓度降低，TNF-α蛋白表达增加，而TGF-β1和HPV16 E2表达降低。ASMq可能改善肝脏中央静脉充血和间质水肿，并维持肝索的放射状结构。
结果表明，ASMq可能降低化疗药物5-氟尿嘧啶治疗宫颈癌时的毒性并增强其疗效。
