Faculty of Traditional Uighur Medicine, Xinjiang Medical University, Urumqi 830011, China.
Evid Based Complement Alternat Med. 2012;2012:926329. doi: 10.1155/2012/926329. Epub 2011 Jul 7.
Aims. Study the effect of Abnormal Savda Munziq (ASMq) ethanol extract on the proliferation, apoptosis, and correlative gene, expression in colon cancer cells (Caco-2) to elucidate the molecular mechanisms responsible for the anticancer property of Abnormal Savda Munziq. Materials and Methods. ASMq ethanol extract was prepared by a professional pharmacist. Caco-2 cells were treated with different concentration of ASMq ethanol extract (0.5-7.5 mg/mL) for different time intervals (48 and 72 h). Antiproliferative effect of ASMq ethanol extract was determined by MTT assay; DNA fragmentation was determined by gel electrophoresis assay; cell cycle analysis was detected by flow cytometer; apoptosis-related gene expression was detected by RT-PCR assay. Results. ASMq ethanol extract possesses an inhibition effect on Caco-2 cells proliferation, induction of cell apoptosis, cell cycle arrest in sub-G1 phase, and downregulation of bcl-2 and upregulation of Bax gene expression. Conclusion. The anticancer mechanism of ASMq ethanol extract may be involved in antiproliferation, induction of apoptosis, cell cycle arrest, and regulation of apoptosis-related gene expression such as bcl-2 and Bax activity pathway.
目的。研究异常黑穗醋栗（ASMq）乙醇提取物对结肠癌细胞（Caco-2）增殖、凋亡及相关基因表达的影响，阐明异常黑穗醋栗抗肿瘤作用的分子机制。
材料和方法。ASMq 乙醇提取物由专业药剂师制备。用不同浓度的 ASMq 乙醇提取物（0.5-7.5mg/ml）处理 Caco-2 细胞不同时间（48 和 72 小时）。用 MTT 法测定 ASMq 乙醇提取物的抗增殖作用；用凝胶电泳法测定 DNA 片段化；用流式细胞仪检测细胞周期；用 RT-PCR 法检测凋亡相关基因表达。
结果。ASMq 乙醇提取物对 Caco-2 细胞增殖具有抑制作用，诱导细胞凋亡，细胞周期阻滞于 sub-G1 期，下调 bcl-2 基因表达，上调 Bax 基因表达。
结论。ASMq 乙醇提取物的抗癌机制可能涉及抑制增殖、诱导凋亡、细胞周期阻滞以及调节凋亡相关基因表达，如 bcl-2 和 Bax 活性途径。
