Low V L, Takaoka H, Adler P H, Ya'cob Z, Norma-Rashid Y, Chen C D, Sofian-Azirun M
Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Department of Agricultural and Environmental Sciences, Clemson University, Clemson, SC, U.S.A.
Med Vet Entomol. 2015 Sep;29(3):330-7. doi: 10.1111/mve.12120. Epub 2015 May 13.
A multi-locus approach was used to examine the DNA sequences of 10 nominal species of blackfly in the Simulium subgenus Gomphostilbia (Diptera: Simuliidae) in Malaysia. Molecular data were acquired from partial DNA sequences of the mitochondria-encoded cytochrome c oxidase subunit I (COI), 12S rRNA and 16S rRNA genes, and the nuclear-encoded 18S rRNA and 28S rRNA genes. No single gene, nor the concatenated gene set, resolved all species or all relationships. However, all morphologically established species were supported by at least one gene. The multi-locus sequence analysis revealed two distinct evolutionary lineages, conforming to the morphotaxonomically recognized Simulium asakoae and Simulium ceylonicum species groups.
采用多位点方法对马来西亚戈姆氏蚋亚属（双翅目：蚋科）10种名义上的蚋的DNA序列进行了研究。分子数据取自线粒体编码的细胞色素c氧化酶亚基I（COI）、12S rRNA和16S rRNA基因以及核编码的18S rRNA和28S rRNA基因的部分DNA序列。没有单个基因或串联基因集能够解析所有物种或所有关系。然而，所有形态学上确定的物种都得到了至少一个基因的支持。多位点序列分析揭示了两个不同的进化谱系，与形态分类学上认可的朝子蚋和锡兰蚋物种组一致。
