Center for Applied Chemical Research, Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
Center for Applied Chemical Research, Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China; State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
Biomaterials. 2015 Aug;59:116-24. doi: 10.1016/j.biomaterials.2015.04.039. Epub 2015 May 15.
Porous nanorods of ceria (PN-Ceria), a novel ceria nanostructure with a large surface area and a high surface Ce(3+) fraction, exhibited strong intrinsic peroxidase activity toward a classical peroxidase substrate in the presence of H2O2. Peroxidase-like activity of ceria originated from surface Ce(3+) species as the catalytic center, thereby explaining the high performance of PN-Ceria as an artificial enzyme mimicking peroxidase. Compared with the natural enzyme horseradish peroxidase (HRP), PN-Ceria showed several advantages such as low cost, easy storage, high sensitivity, and, prominently, chemical and catalytic stability under harsh conditions. Importantly, the enzymatic activity of PN-Ceria remained nearly constant and stable over a wide range of temperature and pH values, ensuring the accuracy and reliability of measurements of its peroxidase-like activity. A PN-Ceria based novel diagnostic system was developed for breast cancer detection with a higher sensitivity than the standard HRP detection system. Our work has laid a solid foundation for the development of PN-Ceria as a novel diagnostic tool for clinical use.
具有大表面积和高表面 Ce(3+)分数的新型氧化铈纳米结构 - 多孔纳米棒（PN-Ceria），在存在 H2O2 的情况下，对经典过氧化物酶底物表现出强的固有过氧化物酶活性。过氧化物酶活性源于表面 Ce(3+)物种作为催化中心，从而解释了 PN-Ceria 作为模拟过氧化物酶的人工酶的高性能。与天然酶辣根过氧化物酶（HRP）相比，PN-Ceria 具有成本低、易于储存、灵敏度高、以及在恶劣条件下具有化学和催化稳定性等优点。重要的是，PN-Ceria 的酶活性在很宽的温度和 pH 值范围内几乎保持不变和稳定，确保了其过氧化物酶样活性测量的准确性和可靠性。已经开发了基于 PN-Ceria 的新型诊断系统用于乳腺癌检测，其灵敏度高于标准 HRP 检测系统。我们的工作为 PN-Ceria 作为临床使用的新型诊断工具的开发奠定了坚实的基础。
