Henan University of Science and Technology, Luoyang 471023, P.R. China.
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research, Institute Chinese Academy of Agricultural Sciences, Harbin 150069, P.R. China.
Poult Sci. 2020 Jun;99(6):3015-3019. doi: 10.1016/j.psj.2020.03.006. Epub 2020 Mar 24.
To evaluate the ability of inulin to enhance the immune response of a ptfA gene DNA vaccine for avian Pasteurella multocida, inulin was added as an adjuvant to the ptfA-DNA vaccine, obtaining an inulin-adjuvant DNA vaccine. The DNA vaccine was administered to chickens; a fimbria protein vaccine and an attenuated live vaccine were used as positive controls. The levels of the serum antibody and concentrations of interferon-γ (IFN-γ), interleukin-2 (IL-2), and interleukin-4 (IL-4) were determined, and a lymphocyte proliferation assay was performed. After being challenged with virulent P. multocida, the protective efficacy was evaluated. The results showed that the serum antibodies induced by the ptfA-DNA vaccine were not enhanced by inulin. The stimulation index values and the concentrations of IL-2 and IFN-γ in chickens vaccinated with inulin-adjuvant DNA vaccine were significantly higher than those in chickens vaccinated with the DNA vaccine, those with the fimbria protein vaccine, and the chickens gavaged with inulin. The concentrations of IL-4 in the inulin-adjuvant DNA vaccine group and the fimbria protein vaccine group were higher than those in the DNA vaccine group and the inulin-gavage group. The protective efficacy rates of the attenuated live vaccine group, the fimbria protein vaccine group, the DNA vaccine group, the inulin-adjuvant DNA vaccine group, and the inulin-gavage group were 90, 70, 55, 65, and 55%, respectively.
为了评估菊糖增强禽多杀性巴氏杆菌 ptfA 基因 DNA 疫苗免疫反应的能力，将菊糖作为佐剂添加到 ptfA-DNA 疫苗中，得到菊糖佐剂 DNA 疫苗。将 DNA 疫苗接种给鸡，以菌毛蛋白疫苗和弱毒活疫苗作为阳性对照。测定血清抗体水平和干扰素-γ（IFN-γ）、白细胞介素-2（IL-2）和白细胞介素-4（IL-4）的浓度，并进行淋巴细胞增殖试验。攻毒后评估保护效力。结果表明，菊糖不能增强 ptfA-DNA 疫苗诱导的血清抗体。接种菊糖佐剂 DNA 疫苗的鸡的刺激指数值和 IL-2、IFN-γ浓度明显高于接种 DNA 疫苗、菌毛蛋白疫苗和菊糖灌胃的鸡。菊糖佐剂 DNA 疫苗组和菌毛蛋白疫苗组的 IL-4 浓度高于 DNA 疫苗组和菊糖灌胃组。弱毒活疫苗组、菌毛蛋白疫苗组、DNA 疫苗组、菊糖佐剂 DNA 疫苗组和菊糖灌胃组的保护效力分别为 90%、70%、55%、65%和 55%。
