Department of Clinical Laboratory, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830011, Xinjiang, China.
Department of Clinical Laboratory, The People Hospital of Xinjiang, Urumqi, 830001, Xinjiang, China.
Sci Rep. 2019 Mar 5;9(1):3512. doi: 10.1038/s41598-019-40084-w.
Given brucellosis is a widespread zoonosis in the world, a safe and effective vaccine is urgently needed. Recent trend in vaccine design has shifted to epitope-based vaccines that are safe and specific. In this study, peptide containing both T-cell and B-cell epitopes of OMP31 was synthesized and used to immunize the mice by nasal administration. The protective efficacy was evaluated. Mice immunized with the B epitope or TB epitope peptides of OMP31 had higher levels of IgG1 and IgG2a in the serum. While the BALB/c mice immunized with peptides containing T cell epitope or TB epitope of OMP31 showed high degree of IFN-γ-producing T cells in the lymphocytes from the respiratory draining lymph nodes and spleen. After intranasally challenged with 5 × 10 CFU of Brucella melitensis (strain 16 M), the bacterial loads in lung of the immunized mice were significantly lower than control group. These data demonstrate for the first time that peptides of OMP31 containing T epitope, B epitope or TB epitopes are of high immunogenicity and thus can protect host from Brucella melitensis infection in lung.
鉴于布鲁氏菌病是世界范围内广泛存在的动物源性传染病，因此迫切需要一种安全有效的疫苗。最近的疫苗设计趋势已经转向基于表位的疫苗，这些疫苗既安全又具有特异性。在这项研究中，合成了含有 OMP31 的 T 细胞和 B 细胞表位的肽，并通过鼻内给药对小鼠进行免疫。评估了保护效果。用 OMP31 的 B 表位或 TB 表位肽免疫的小鼠在血清中具有更高水平的 IgG1 和 IgG2a。而用含有 OMP31 的 T 细胞表位或 TB 表位肽的 BALB/c 小鼠，其呼吸道引流淋巴结和脾脏中的淋巴细胞中产生 IFN-γ的 T 细胞数量较高。经鼻腔感染 5×10 CFU 布鲁氏菌（菌株 16M）后，免疫小鼠肺部的细菌载量明显低于对照组。这些数据首次表明，含有 T 表位、B 表位或 TB 表位的 OMP31 肽具有高度的免疫原性，因此可以保护宿主免受肺部布鲁氏菌感染。
