Park Sooyeon, Park Ji-Min, Won Sung-Min, Park Doo-Sang, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, South Korea.
Int J Syst Evol Microbiol. 2015 Aug;65(8):2689-2695. doi: 10.1099/ijs.0.000324. Epub 2015 May 12.
A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and ovoid or rod-shaped bacterial strain, designated TYO-8T, was isolated from an oyster collected from the South Sea in South Korea. Strain TYO-8T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0% (w/v) NaCl. A neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, revealed that strain TYO-8T fell within the clade comprising the type strains of species of the genus Lutibacter, clustering coherently with the type strain of Lutibacter litoralis with a sequence similarity of 99.3%. Strain TYO-8T exhibited 16S rRNA gene sequence similarity values of 95.3-97.5% to the type strains of other species of the genus Lutibacter and of less than 92.9% to the type strains of other species with validly published names. Strain TYO-8T contained MK-6 as the predominant menaquinone and iso-C15 : 0, iso-C15 : 0 3-OH and iso-C15 : 1 G as the major fatty acids. The major polar lipids of strain TYO-8T were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain TYO-8T was 33.8 mol% and its DNA-DNA relatedness values with the type strains of L. litoralis, Lutibacter aestuarii and Lutibacter flavus were 13-27%. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain TYO-8T is distinct from other species of the genus Lutibacter. On the basis of the data presented, strain TYO-8T is considered to represent a novel species of the genus Lutibacter, for which the name Lutibacter crassostreae sp. nov. is proposed. The type strain is TYO-8T ( = KCTC 42461T = NBRC 110923T).
从韩国南海采集的一只牡蛎中分离出一株革兰氏染色阴性、需氧、不形成芽孢、无鞭毛、不滑动且呈卵形或杆状的细菌菌株，命名为TYO-8T。菌株TYO-8T在25 °C、pH 7.0 - 8.0以及2.0 - 3.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法系统发育树显示，菌株TYO-8T属于包含Lutibacter属各物种模式菌株的进化分支，与Lutibacter litoralis模式菌株紧密聚类，序列相似性为99.3%。菌株TYO-8T与Lutibacter属其他物种的模式菌株的16S rRNA基因序列相似性值为95.3 - 97.5%，与其他有效发表名称的物种的模式菌株的相似性值小于92.9%。菌株TYO-8T以MK-6作为主要甲基萘醌，以iso-C15 : 0、iso-C15 : 0 3-OH和iso-C15 : 1 G作为主要脂肪酸。菌株TYO-8T的主要极性脂质为磷脂酰乙醇胺和两种未鉴定的脂质。菌株TYO-8T的DNA G+C含量为33.8 mol%，其与L. litoralis、Lutibacter aestuarii和Lutibacter flavus模式菌株的DNA-DNA相关性值为13 - 27%。这些不同的表型特征，连同系统发育和遗传独特性，表明菌株TYO-8T与Lutibacter属的其他物种不同。根据所提供的数据，菌株TYO-8T被认为代表Lutibacter属的一个新物种，为此提出Lutibacter crassostreae sp. nov.这个名称。模式菌株为TYO-8T（ = KCTC 42461T = NBRC 110923T）。
