Kumar Pradeep, Singh V K, Singh D K
Malacology Laboratory, Department of Zoology, Deen Dayal Upadhyay Gorakhpur University, Gorakhpur 273009, India.
ISRN Biochem. 2012 Dec 4;2012:343047. doi: 10.5402/2012/343047. eCollection 2012.
Fascioliasis, a snail-borne parasitic zoonosis, has been recognized for a long time because of its major veterinary and human impact. Different Bait formulations were fed to the snail Lymnaea acuminata in clear glass aquaria having diameter of 30 cm. Snail attractant containing bait formulations was prepared from different binary combination (1 : 1 ratio) of carbohydrates (glucose, starch 10 mM) and amino acid (methionine, histidine 10 mM) in 100 ml of 2% agar solution + sublethal (20% and 60% of 24 h and 96 h LC50) doses of different molluscicides (eugenol, ferulic acid, umbelliferone, and limonene). Snails fed on bait containing sub-lethal concentration of different molluscicides and the snail attractant, causing a significant inhibition in alkaline phosphatase (ALP) and acetylcholinesterase (AChE) activity in the nervous tissue of the vector snail L. acuminata. Maximum inhibition in ALP (20% of control) and AChE (49.49% of control) activity was observed in the nervous tissue of the L. acuminata exposed to 60% of 96 h LC50 of eugenol in the bait pellets containing starch + histidine, starch + methionine, respectively.
肝片吸虫病是一种由蜗牛传播的寄生性人畜共患病，因其对兽医和人类的重大影响，早已为人所知。在直径为30厘米的透明玻璃水族箱中，将不同的诱饵配方投喂给尖膀胱螺。含有诱饵配方的蜗牛引诱剂由碳水化合物（葡萄糖、10 mM淀粉）和氨基酸（10 mM蛋氨酸、组氨酸）以不同的二元组合（1∶1比例），在100毫升2%琼脂溶液中 + 不同杀软体动物剂（丁香酚、阿魏酸、伞形酮和柠檬烯）的亚致死剂量（24小时和96小时LC50的20%和60%）制备而成。蜗牛取食含有不同杀软体动物剂亚致死浓度和蜗牛引诱剂的诱饵后，对中间宿主螺尖膀胱螺神经组织中的碱性磷酸酶（ALP）和乙酰胆碱酯酶（AChE）活性产生显著抑制。在分别含有淀粉 + 组氨酸、淀粉 + 蛋氨酸的诱饵颗粒中，暴露于96小时LC50的60%丁香酚的尖膀胱螺神经组织中，观察到ALP活性的最大抑制（对照的20%）和AChE活性的最大抑制（对照的49.49%）。
