Anal Chem. 2015 Jun 16;87(12):5891-6. doi: 10.1021/acs.analchem.5b00287. Epub 2015 May 22.
Here, we reported a strategy-based plasmonic enzyme-linked immunosorbent assay (ELISA) using alcohol dehydrogenase-catalyzed gold nanoparticle seed-mediated growth to serve as a colorimetric signal generation method for detecting disease biomarkers with the naked eye. This system possesses the advantages of outstanding robustness, sensitivity, and universality. By using this strategy, we investigated the hepatitis B surface antigen (HBsAg) and α-fetoprotein (AFP) with the lowest concentration of naked-eye detection down to 1.0 × 10(-12) g mL(-1). Experiments with real serum samples from HBsAg-infected patients are presented, demonstrating the potential for clinical analysis. Our method eliminates the need for sophisticated instruments and high detection expenses, making it possible to be a reliable alternative in resource-constrained regions.
在这里，我们报告了一种基于策略的等离子体酶联免疫吸附测定（ELISA），使用醇脱氢酶催化的金纳米粒子种子介导的生长作为比色信号产生方法，用于通过肉眼检测疾病生物标志物。该系统具有出色的稳健性、灵敏度和通用性的优点。通过使用这种策略，我们以 1.0×10(-12) g mL(-1)的最低浓度检测到乙型肝炎表面抗原（HBsAg）和甲胎蛋白（AFP），达到了肉眼检测的水平。我们用来自 HBsAg 感染患者的真实血清样本进行了实验，证明了其在临床分析中的潜力。我们的方法消除了对复杂仪器和高检测费用的需求，使其成为资源有限地区的可靠替代方法。
