Department of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, Santariskiu 5, LT-08406 Vilnius, Lithuania.
Biomedical Physics Laboratory, National Cancer Institute, Baublio 3b, LT-08406 Vilnius, Lithuania.
Sensors (Basel). 2020 Dec 2;20(23):6890. doi: 10.3390/s20236890.
The ever-growing demand for fast, cheap, and reliable diagnostic tools for personalised medicine is encouraging scientists to improve existing technology platforms and to create new methods for the detection and quantification of biomarkers of clinical significance. Simultaneous detection of multiple analytes allows more accurate assessment of changes in biomarker expression and offers the possibility of disease diagnosis at the earliest stages. The concept of multiplexing, where multiple analytes can be detected in a single sample, can be tackled using several types of nanomaterial-based biosensors. Quantum dots are widely used photoluminescent nanoparticles and represent one of the most frequent choices for different multiplex systems. However, nanoparticles that incorporate gold, silver, and rare earth metals with their unique optical properties are an emerging perspective in the multiplexing field. In this review, we summarise progress in various nanoparticle applications for multiplexed biomarkers.
对于用于个性化医疗的快速、廉价且可靠的诊断工具的需求不断增长，这促使科学家改进现有技术平台并创造新方法来检测和定量具有临床意义的生物标志物。同时检测多个分析物可以更准确地评估生物标志物表达的变化，并有可能在疾病的最早阶段进行诊断。多重检测的概念，即在单个样本中可以同时检测多个分析物，可以通过几种基于纳米材料的生物传感器来实现。量子点是广泛使用的发光明纳米粒子，是不同多重系统中最常见的选择之一。然而，具有独特光学特性的金、银和稀土金属等纳米粒子在多重检测领域是一个新兴的研究方向。在这篇综述中，我们总结了各种纳米粒子在多重生物标志物检测中的应用进展。
