从日本黑兔组织提取物中分离出的新型二肽β-天冬氨酰甘氨酸中D-天冬氨酸的确认。
Confirmation of D-aspartic acid in the novel dipeptide beta-aspartylglycine isolated from tissue extract of Aplysia kurodai.
作者信息
Sato M, Yamaguchi T, Kanno N, Sato Y
机构信息
School of Fisheries Sciences, Kitasato University, Iwate, Japan.
出版信息
Biochem J. 1989 Oct 15;263(2):617-20. doi: 10.1042/bj2630617.
A novel o-phthalaldehyde-reactive compound was found in the h.p.l.c. chromatogram of Aplysia kurodai extract. This compound was isolated by ion-exchange chromatography and preparative high-voltage paper electrophoresis. It was shown by optical-rotatory-dispersion spectrum and optical-resolution h.p.l.c. analysis that this compound consisted of equimolar amounts of D-aspartic acid and glycine. This compound resisted cleavage in the Edman reaction. This peptide was inferred to be beta-D-aspartylglycine, and this was confirmed by synthesis. beta-D-Aspartylglycine was detected in all tissues of Aplysia kurodai, with especially high concentrations in body wall (skin and muscle) and gill.
在黑指纹海兔提取物的高效液相色谱图中发现了一种新型邻苯二甲醛反应性化合物。该化合物通过离子交换色谱法和制备型高压纸电泳法分离得到。旋光色散光谱和光学拆分高效液相色谱分析表明，该化合物由等摩尔量的D-天冬氨酸和甘氨酸组成。该化合物在埃德曼反应中抗裂解。推测该肽为β-D-天冬氨酰甘氨酸，合成实验证实了这一推测。在黑指纹海兔的所有组织中均检测到β-D-天冬氨酰甘氨酸，在体壁（皮肤和肌肉）和鳃中的浓度尤其高。
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