Zhao Hua, Li Ke, Tang Jia-Yong, Zhou Ji-Chang, Wang Kang-Ning, Xia Xin-Jie, Lei Xin Gen
International Center of Future Agriculture for Human Health, Sichuan Agricultural University, Sichuan, China;
Molecular Biology Laboratory, Shenzhen Center for Chronic Disease Control, Shenzhen, China;
J Nutr. 2015 Jul;145(7):1394-401. doi: 10.3945/jn.115.211318. Epub 2015 May 13.
Relations of the 25 mammalian selenoprotein genes with obesity and the associated inflammation remain unclear.
This study explored impacts of high-fat diet-induced obesity on inflammation and expressions of selenoprotein and obesity-related genes in 10 tissues of pigs.
Plasma and 10 tissues were collected from pigs (n = 10) fed a corn-soy-based control diet or that diet containing 3-7% lard from weanling to finishing (180 d). Plasma concentrations (n = 8) of cytokines and thyroid hormones and tissue mRNA abundance (n = 4) of 25 selenoprotein genes and 16 obesity-related genes were compared between the pigs fed the control and high-fat diets. Stepwise regression was applied to analyze correlations among all these measures, including the previously reported body physical and plasma biochemical variables.
The high-fat diet elevated (P < 0.05) plasma concentrations of tumor necrosis factor α, interleukin-6, leptin, and leptin receptor by 29-42% and affected (P < 0.05-0.1) tissue mRNA levels of the selenoprotein and obesity-related genes in 3 patterns. Specifically, the high-fat diet up-regulated 12 selenoprotein genes in 6 tissues, down-regulated 13 selenoprotein genes in 7 tissues, and exerted no effect on 5 genes in any tissue. Body weights and plasma triglyceride concentrations of pigs showed the strongest regressions to tissue mRNA abundances of selenoprotein and obesity-related genes. Among the selenoprotein genes, selenoprotein V and I were ranked as the strongest independent variables for the regression of phenotypic and plasma measures. Meanwhile, agouti signaling protein, adiponectin, and resistin genes represented the strongest independent variables of the obesity-related genes for the regression of tissue selenoprotein mRNA.
The high-fat diet induced inflammation in pigs and affected their gene expression of selenoproteins associated with thioredoxin and oxidoreductase systems, local tissue thyroid hormone activity, endoplasmic reticulum protein degradation, and phosphorylation of lipids. This porcine model may be used to study interactive mechanisms between excess fat intake and selenoprotein function.
25种哺乳动物硒蛋白基因与肥胖及相关炎症之间的关系尚不清楚。
本研究探讨高脂饮食诱导的肥胖对猪10种组织中炎症、硒蛋白及肥胖相关基因表达的影响。
从断奶至育肥（180天）期间，给猪（n = 10）饲喂基于玉米-大豆的对照日粮或含3-7%猪油的日粮，采集血浆和10种组织。比较对照日粮组和高脂日粮组猪的细胞因子和甲状腺激素血浆浓度（n = 8）以及25种硒蛋白基因和16种肥胖相关基因的组织mRNA丰度（n = 4）。应用逐步回归分析所有这些指标之间的相关性，包括先前报道的身体物理和血浆生化变量。
高脂日粮使肿瘤坏死因子α、白细胞介素-6、瘦素和瘦素受体的血浆浓度升高（P < 0.05）29%-42%，并以3种模式影响（P < 0.05-0.1）硒蛋白和肥胖相关基因的组织mRNA水平。具体而言，高脂日粮上调了6种组织中的12种硒蛋白基因，下调了7种组织中的13种硒蛋白基因，对任何组织中的5种基因均无影响。猪的体重和血浆甘油三酯浓度与硒蛋白和肥胖相关基因的组织mRNA丰度呈最强的回归关系。在硒蛋白基因中，硒蛋白V和I是表型和血浆指标回归的最强独立变量。同时，刺鼠信号蛋白、脂联素和抵抗素基因是组织硒蛋白mRNA回归中肥胖相关基因的最强独立变量。
高脂日粮诱导猪发生炎症，并影响其与硫氧还蛋白和氧化还原酶系统、局部组织甲状腺激素活性、内质网蛋白降解及脂质磷酸化相关的硒蛋白基因表达。该猪模型可用于研究过量脂肪摄入与硒蛋白功能之间的相互作用机制。
