Lavrnja Irena, Parabucki Ana, Brkic Predrag, Jovanovic Tomislav, Dacic Sanja, Savic Danijela, Pantic Igor, Stojiljkovic Mirjana, Pekovic Sanja
Department of Neurobiology, Institute for Biological Research "Sinisa Stankovic", University of Belgrade, 11060 Belgrade, Serbia.
Institute of Medical Physiology "Richard Burian", School of Medicine, University of Belgrade, 11000 Belgrade, Serbia.
Mediators Inflamm. 2015;2015:498405. doi: 10.1155/2015/498405. Epub 2015 Apr 20.
The exact mechanisms by which treatment with hyperbaric oxygen (HBOT) exerts its beneficial effects on recovery after brain injury are still unrevealed. Therefore, in this study we investigated the influence of repetitive HBOT on the reactive astrogliosis and expression of mediators of inflammation after cortical stab injury (CSI). CSI was performed on male Wistar rats, divided into control, sham, and lesioned groups with appropriate HBO. The HBOT protocol was as follows: 10 minutes of slow compression, 2.5 atmospheres absolute (ATA) for 60 minutes, and 10 minutes of slow decompression, once a day for 10 consecutive days. Data obtained using real-time polymerase chain reaction, Western blot, and immunohistochemical and immunofluorescence analyses revealed that repetitive HBOT applied after the CSI attenuates reactive astrogliosis and glial scarring, and reduces expression of GFAP (glial fibrillary acidic protein), vimentin, and ICAM-1 (intercellular adhesion molecule-1) both at gene and tissue levels. In addition, HBOT prevents expression of CD40 and its ligand CD40L on microglia, neutrophils, cortical neurons, and reactive astrocytes. Accordingly, repetitive HBOT, by prevention of glial scarring and limiting of expression of inflammatory mediators, supports formation of more permissive environment for repair and regeneration.
高压氧治疗(HBOT)对脑损伤后恢复产生有益作用的确切机制仍未明确。因此,在本研究中,我们调查了重复HBOT对皮质刺伤(CSI)后反应性星形胶质细胞增生和炎症介质表达的影响。对雄性Wistar大鼠进行CSI,并将其分为对照组、假手术组和接受适当高压氧治疗的损伤组。HBOT方案如下:缓慢加压10分钟,绝对压力2.5个大气压(ATA)持续60分钟,然后缓慢减压10分钟,每天1次,连续10天。使用实时聚合酶链反应、蛋白质印迹以及免疫组织化学和免疫荧光分析获得的数据显示,CSI后应用重复HBOT可减轻反应性星形胶质细胞增生和胶质瘢痕形成,并在基因和组织水平上降低胶质纤维酸性蛋白(GFAP)、波形蛋白和细胞间黏附分子-1(ICAM-1)的表达。此外,HBOT可抑制小胶质细胞、中性粒细胞、皮质神经元和反应性星形胶质细胞上CD40及其配体CD40L的表达。因此,重复HBOT通过防止胶质瘢痕形成和限制炎症介质的表达,为修复和再生支持形成更有利的环境。