Mathews Smitha, Lakshmi Rao K, Suma Prasad K, Kanakavalli M K, Govardhana Reddy A, Avinash Raj T, Thangaraj Kumarasamy, Pande Gopal
CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India.
1] Sridevi Nursing Home, Warasiguda, Hyderabad 500361, India [2] Prasad Hospital and Research Centre, Nacharam, Hyderabad 500076, India.
Sci Rep. 2015 May 15;5:10054. doi: 10.1038/srep10054.
Long term propagation of human fetal mesenchymal stromal cells (MSC) in vitro has proven elusive due to limited availability of fetal tissue sources and lack of appropriate methodologies. Here, we have demonstrated the presence of fetal and maternal cells within the tips of terminal chorionic villi (TCV) of normal human term placenta, and we have exploited inherent differences in the adhesive and migratory properties of maternal vs. fetal cells, to establish pure MSC cultures of both cell types. The origin and purity of each culture was confirmed by X-Y chromosome-specific fluorescence in situ hybridization (FISH) and short tandem repeat (STR) genotyping. This is the first demonstration of fetal and maternal cells in the TCV of human term placenta and also of deriving pure fetal MSC cultures from them. The concomitant availability of pure cultures of adult and fetal MSC from one tissue provides a good system to compare genetic and epigenetic differences between adult and fetal MSCs; and also to generate new models of cell based therapies in regenerative medicine.
由于胎儿组织来源有限且缺乏合适的方法，人胎儿间充质基质细胞（MSC）的长期体外培养一直难以实现。在此，我们已证实在正常足月人胎盘的终末绒毛尖端（TCV）存在胎儿和母体细胞，并且我们利用母体与胎儿细胞在黏附及迁移特性上的内在差异，建立了两种细胞类型的纯MSC培养物。通过X - Y染色体特异性荧光原位杂交（FISH）和短串联重复序列（STR）基因分型确认了每种培养物的来源和纯度。这是首次在足月人胎盘的TCV中证实胎儿和母体细胞的存在，也是首次从它们中获得纯胎儿MSC培养物。从同一组织中同时获得成人和胎儿MSC的纯培养物，为比较成人和胎儿MSC之间的遗传和表观遗传差异提供了一个良好的系统；也为再生医学中基于细胞的治疗生成新模型。
