Zhu Yan, Li Cheng-Shan, Wang Yuan-Ye, Zhou Sheng-Nian
Department of ICU, Zaozhuang Municipal Hospital (Jining medical college affiliated hospital), Zaozhuang, Shandong, P. R. China.
Department of ICU, Zaozhuang Municipal Hospital (Jining medical college affiliated hospital), Zaozhuang, Shandong, P. R. China.
Asian Pac J Trop Med. 2015 Apr;8(4):292-8. doi: 10.1016/S1995-7645(14)60333-3.
To To investigate the changes of MicroRNA-134, CREB and p-CREB expression in epileptic rat brains in order to elucidate the molecular mechanisms of epilepsy, providing new ideas for clinical treatment.
Sixty-four Spraque-Dawley (SD) rats were divided into groups randomly, including control group, six hours after seizure group, 24-hour group, three-day group, one-week group, two-week group, four-week group, and eight-week group. All groups were placed under a pilocarpine-induced epilepsy model except the control group, and all rats were decapitated in different points of time. Brain specimens were taken for quantitative PCR experiments, immunohistochemistry and Western blot experiments. The results of the epilepsy model groups and the control group were compared.
There were no significant differences between the six hours after seizure group, the 24-hour group and the control group about the MicroRNA-134 levels. MicroRNA-134 in the hippocampus tissue of the three-day group significantly reduced compared with the control group; same result was observed with the one-week, two-week, four-week and eight-week groups. The CREB and p-CREB levels in the three-day group's rat hippocampus significantly increased compared with the control group; and the high levels of CREB and p-CREB were constantly maintained in the one-week, two-week, four-week and eight-week groups.
The MicroRNA-134 level of the epileptic rat hippocampus is significantly lower than normal after three days, and continues to maintain a low level; while CREB and p-CREB levels are rsignificantly increased after three days, and continue to remain at a high level. MicroRNA-134 plays a role in inhibiting synaptic plasticity by inhibiting CREB and p-CREB expressions.
研究癫痫大鼠脑内微小RNA-134、CREB及p-CREB表达变化，以阐明癫痫发病的分子机制，为临床治疗提供新思路。
将64只Spraque-Dawley（SD）大鼠随机分组，包括对照组、癫痫发作后6小时组、24小时组、3天组、1周组、2周组、4周组和8周组。除对照组外，其余各组均采用匹罗卡品诱导建立癫痫模型，在不同时间点断头处死大鼠，取脑标本进行定量PCR实验、免疫组化及蛋白质印迹实验，比较癫痫模型组与对照组结果。
癫痫发作后6小时组、24小时组与对照组微小RNA-134水平差异无统计学意义。3天组海马组织微小RNA-134较对照组显著降低；1周组、2周组、4周组和8周组结果相同。3天组大鼠海马CREB及p-CREB水平较对照组显著升高；1周组、2周组、4周组和8周组持续维持较高水平。
癫痫大鼠海马微小RNA-134水平在3天后显著低于正常水平，并持续维持低水平；而CREB及p-CREB水平在3天后显著升高，并持续维持高水平。微小RNA-134可能通过抑制CREB及p-CREB表达发挥抑制突触可塑性的作用。
