Nam Sun-Young, Kim Hee-Yun, Yoou Myoung-Schook, Kim A Hyun, Park Byoung Jun, Jeong Hyun-Ja, Kim Hyung-Min
Department of Pharmacology, College of Korean Medicine, Kyung Hee University , Seoul , Republic of Korea .
Immunopharmacol Immunotoxicol. 2015 Jun;37(3):258-64. doi: 10.3109/08923973.2015.1026604. Epub 2015 May 15.
Isoacteoside, a dihydroxypheynylethyl glycoside, is a major bioactive component of Abeliophyllum distichum (White Forsythia) which is a deciduous shrub native to the south and central areas of Korea. The present study is designed to evaluate the anti-inflammatory activities and underlying mechanisms of isoacteoside in human mast cell line, HMC-1 cells. We isolated isoacteoside from A. distichum. The anti-inflammatory effect of isoacteoside was investigated in HMC-1 cells by studying the following markers: phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-induced interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor alpha (TNF-α) secretion and mRNA expression by ELISA and RT-PCR, respectively. In addition, mechanism related to anti-inflammatory was investigated by Western blotting. Isoacteoside significantly suppressed the production and mRNA expression of proinflammatory cytokines including IL-1β, IL-6, IL-8 and TNF-α in PMACI-stimulated HMC-1 cells without cytotoxicity. It was found that anti-inflammatory effects of isoacteoside are mediated by action on caspase-1, mitogen-activated protein kinases (c-Jun N-terminal kinase, p38, extracellular signal-regulated protein kinase) and nuclear factor-kappa B pathways. Taken together, the present findings provide new insights that isoacteoside may be a promising anti-inflammatory agent for inflammatory disorders.
异毛蕊花糖苷是一种二羟基苯乙基糖苷，是毛叶山珊瑚（白连翘）的主要生物活性成分，毛叶山珊瑚是一种原产于韩国南部和中部地区的落叶灌木。本研究旨在评估异毛蕊花糖苷在人肥大细胞系HMC-1细胞中的抗炎活性及其潜在机制。我们从毛叶山珊瑚中分离出异毛蕊花糖苷。通过研究以下标志物，在HMC-1细胞中研究了异毛蕊花糖苷的抗炎作用：佛波醇12-肉豆蔻酸酯13-乙酸酯和钙离子载体A23187（PMACI）诱导的白细胞介素（IL）-1β、IL-6、IL-8和肿瘤坏死因子α（TNF-α）的分泌及mRNA表达，分别采用酶联免疫吸附测定（ELISA）和逆转录-聚合酶链反应（RT-PCR）法。此外，通过蛋白质免疫印迹法研究了与抗炎相关的机制。异毛蕊花糖苷显著抑制了PMACI刺激的HMC-1细胞中促炎细胞因子IL-1β、IL-6、IL-8和TNF-α的产生及mRNA表达，且无细胞毒性。发现异毛蕊花糖苷的抗炎作用是通过作用于半胱天冬酶-1、丝裂原活化蛋白激酶（c-Jun氨基末端激酶、p38、细胞外信号调节蛋白激酶）和核因子-κB通路介导的。综上所述，本研究结果提供了新的见解，即异毛蕊花糖苷可能是一种用于炎症性疾病的有前景的抗炎药物。
