Mrdaković Marija, Ilijin Larisa, Vlahović Milena, Todorović Dajana, Gavrilović Anja, Mrkonja Aleksandra, Perić-Mataruga Vesna
Department of Insect Physiology and Biochemistry, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, Belgrade, Serbia,
Environ Sci Pollut Res Int. 2015 Jul;22(13):10367-74. doi: 10.1007/s11356-015-4653-x. Epub 2015 May 16.
This study aimed to examine the effects of ubiquitous polycyclic aromatic hydrocarbon fluoranthene, supplemented to an artificial diet, on the fitness-related traits and activity of midgut antioxidative enzymes-superoxide dismutase (SOD) and catalase (CAT), and expression of their isoforms in the fifth-instar gypsy moth Lymantria dispar L. Prolonged duration of development and reduced weight and relative growth rate were recorded in larvae reared on the diets supplemented with different concentrations of fluoranthene. SOD and CAT activities were significantly higher in the midguts of fluoranthene-treated larvae, compared to that of the control group. Different expression patterns were detected for SOD as well as for CAT isoforms, depending on the supplemented concentration of fluoranthene. Obtained results suggest that the activity of these enzymes in gypsy moth larvae may be used as biomarkers for assessing pollution, even at low concentrations of the pollutant.
本研究旨在探讨在人工饲料中添加普遍存在的多环芳烃荧蒽，对五龄舞毒蛾（Lymantria dispar L.）与 fitness 相关的性状、中肠抗氧化酶超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性及其同工型表达的影响。在添加不同浓度荧蒽的饲料上饲养的幼虫，发育时间延长，体重和相对生长率降低。与对照组相比，荧蒽处理幼虫中肠的 SOD 和 CAT 活性显著更高。根据荧蒽的添加浓度，检测到 SOD 以及 CAT 同工型的不同表达模式。所得结果表明，即使在低浓度污染物情况下，这些酶在舞毒蛾幼虫中的活性也可作为评估污染的生物标志物。
