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Cell adhesion and sorting in embryoid bodies derived from N- or E-cadherin deficient murine embryonic stem cells.胚体中细胞黏附和分选来源于 N- 或 E- 钙黏蛋白缺陷型鼠胚胎干细胞。
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A system to enrich for primitive streak-derivatives, definitive endoderm and mesoderm, from pluripotent cells in culture.一种从培养的多能细胞中富集原始生殖嵴衍生物、内胚层和中胚层的系统。
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Confined 3D microenvironment regulates early differentiation in human pluripotent stem cells.密闭 3D 微环境调控人多能干细胞的早期分化。
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Role of E-cadherin and other cell adhesion molecules in survival and differentiation of human pluripotent stem cells.E-钙黏蛋白和其他细胞黏附分子在人多能干细胞存活和分化中的作用。
Cell Adh Migr. 2012 Jan-Feb;6(1):59-70. doi: 10.4161/cam.19583.
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Pancreatic transcription factors containing protein transduction domains drive mouse embryonic stem cells towards endocrine pancreas.含有蛋白转导结构域的胰腺转录因子可将小鼠胚胎干细胞诱导为内分泌胰腺细胞。
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Heparan sulfate facilitates FGF and BMP signaling to drive mesoderm differentiation of mouse embryonic stem cells.硫酸乙酰肝素促进 FGF 和 BMP 信号转导，从而驱动小鼠胚胎干细胞的中胚层分化。
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Embryonic stem cell bioprinting for uniform and controlled size embryoid body formation.胚胎干细胞生物打印用于均匀且可控大小的类胚体形成。
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Differential requirement for the dual functions of β-catenin in embryonic stem cell self-renewal and germ layer formation.β-catenin 的双重功能在胚胎干细胞自我更新和胚层形成中的差异需求。
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Antagonistic interactions of hedgehog, Bmp and retinoic acid signals control zebrafish endocrine pancreas development. hedgehog、Bmp 和视黄酸信号的拮抗相互作用控制斑马鱼内分泌胰腺的发育。
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胚胎干细胞通过胚状体（EBs）的形成实现神经定向分化。

Neural Commitment of Embryonic Stem Cells through the Formation of Embryoid Bodies (EBs).

作者信息

Liyang Gao, Abdullah Syahril, Rosli Rozita, Nordin Norshariza

机构信息

Department of Obstetrics & Gynaecology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia ; Genetics & Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

Department of Obstetrics & Gynaecology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia ; Genetics & Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia ; UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience, 43400 UPM Serdang, Selangor, Malaysia.

出版信息

Malays J Med Sci. 2014 Sep-Oct;21(5):8-16.

PMID:25977628
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4418120/
Abstract

An embryonic stem cell (ESC) is a good tool to generate neurons in vitro and can be used to mimic neural development in vivo. It has been widely used in research to examine the role of cell signalling during neuronal development, test the effects of drugs on neurons, and generate a large population of functional neurons. So far, a number of protocols have been established to promote the differentiation of ESCs, such as direct and indirect differentiation. One of the widely used protocols to generate neurons is through the spontaneous formation of multicellular aggregates known as embryonic bodies (EBs). However, for some, it is not clear why EB protocol could be the protocol of choice. EB also is known to mimic an early embryo; hence, knowing the similarities between EB and an early embryo is essential, particularly the information on the players that promote the formation of EBs or the aggregation of ESCs. This review paper focuses on these issues and discusses further the generation of neural cells from EBs using a well-known protocol, the 4-/4+ protocol.

摘要

胚胎干细胞（ESC）是体外生成神经元的良好工具，可用于模拟体内神经发育。它已广泛应用于研究中，以检查细胞信号传导在神经元发育过程中的作用、测试药物对神经元的影响，以及生成大量功能性神经元。到目前为止，已经建立了许多促进ESC分化的方案，如直接分化和间接分化。生成神经元的广泛使用的方案之一是通过自发形成称为胚胎体（EB）的多细胞聚集体。然而，对于一些人来说，尚不清楚为什么EB方案可能是首选方案。EB也已知可模拟早期胚胎；因此，了解EB与早期胚胎之间的相似性至关重要，特别是关于促进EB形成或ESC聚集的因素的信息。这篇综述文章聚焦于这些问题，并进一步讨论使用著名的4-/4+方案从EB生成神经细胞的情况。